TY - CHAP
T1 - Development and application of classical genetics in toxoplasma gondii
AU - Ajioka, J. W.
AU - Sibley, L. D.
N1 - Funding Information:
We would like to thank the following individuals, who have made important contributions to the advances summarized here: Matt Berriman, John Boothroyd, Jon Boyle, Sandy Clifton, J.P. Dubey, David Ferguson, Martin Fraunholz, Jack Frenkel, Bindu Gajria, Neil Hall, Dan Howe, Krys Kelly, Asis Khan, Jessica Kissinger, Aaron Mackey, Ian Paulsen, Elmer Pfefferkorn, David Roos, Christian Stoeckert Jr, Chunlei Su, Keliang Tang, Sonya Taylor, Michael White, John Wootton, the Genome Sequencing Center at Washington University, The Institute for Genomic Research (TIGR), and the Genomics Institute at the University of Pennsylvania. The National Institutes of Health (USA), United States Department of Agriculture (USA), Burroughs Wellcome Fund (USA), Wellcome Trust (UK), and Biotechnology and Biological Sciences Research Council (UK) have supported work in the authors' laboratories.
PY - 2007
Y1 - 2007
N2 - Toxoplasma gondii is a member of the phylum Apicomplexa: a diverse group of largely parasitic organisms that contains a number of human pathogens. Toxoplasma gondii has a typical heteroxenous life cycle, alternating between sexual replication in members of the cat family, which serve as the definitive host, and asexual replication in a wide range of warm-blooded vertebrates that serve as intermediate hosts. Apicomplexan parasites have complex life cycles involving both asexual and sexual replication in a variety of invertebrate and vertebrate hosts. The extent to which sexual recombination occurs in different apicomplexan species varies greatly: in organisms like Plasmodium it is essential for transmission, while in the case of Toxoplasma gondii and asexual replicating stages can also give rise to infection of naïve hosts. The ability to cross strains experimentally has been exploited to develop conventional genetic mapping strategies in T. gondii. Genetic mapping provides a forward genetic system for analyzing complex biological traits, including drug resistance, growth, virulence, and induction of host immune responses. Classical genetic analysis offers the most promise when combined with other technologies, such as reverse genetics, comparative genomics, and gene expression studies. © 2007
AB - Toxoplasma gondii is a member of the phylum Apicomplexa: a diverse group of largely parasitic organisms that contains a number of human pathogens. Toxoplasma gondii has a typical heteroxenous life cycle, alternating between sexual replication in members of the cat family, which serve as the definitive host, and asexual replication in a wide range of warm-blooded vertebrates that serve as intermediate hosts. Apicomplexan parasites have complex life cycles involving both asexual and sexual replication in a variety of invertebrate and vertebrate hosts. The extent to which sexual recombination occurs in different apicomplexan species varies greatly: in organisms like Plasmodium it is essential for transmission, while in the case of Toxoplasma gondii and asexual replicating stages can also give rise to infection of naïve hosts. The ability to cross strains experimentally has been exploited to develop conventional genetic mapping strategies in T. gondii. Genetic mapping provides a forward genetic system for analyzing complex biological traits, including drug resistance, growth, virulence, and induction of host immune responses. Classical genetic analysis offers the most promise when combined with other technologies, such as reverse genetics, comparative genomics, and gene expression studies. © 2007
UR - http://www.scopus.com/inward/record.url?scp=57449121298&partnerID=8YFLogxK
U2 - 10.1016/B978-012369542-0/50016-7
DO - 10.1016/B978-012369542-0/50016-7
M3 - Chapter
AN - SCOPUS:57449121298
SN - 9780123695420
SP - 367
EP - 389
BT - Toxoplasma Gondii
PB - Elsevier Ltd
ER -