TY - JOUR
T1 - Developing Microelectrode Arrays for the Point-of-Care Multiplex Detection of Metabolites
AU - Chang, Yu Chia
AU - Arnould, Benoit
AU - Heemstra, Jennifer M.
AU - Moeller, Kevin
N1 - Publisher Copyright:
© 2024 American Chemical Society.
PY - 2024/9/10
Y1 - 2024/9/10
N2 - DNA-aptamer-functionalized electrode arrays can provide an intriguing method for detecting pathogen-derived exometabolites. This work addresses the limitations of previous aptamer-based pathogen detection methods by introducing a novel surface design that bridges the gap between initial efforts in this area and the demands of a point-of-care device. Specifically, the use of a diblock copolymer coating on a high-density microelectrode array and Cu-mediated cross coupling reactions that allow for the exclusive functionalization of that coating by any electrode or set of electrodes in the array provides a device that is stable for 1 year and compatible with the multiplex detection of small-molecule targets. The new chemistry developed allows one to take advantage of a large number of electrodes in the array with one experiment described herein capitalizing on the use of 960 individually addressable electrodes.
AB - DNA-aptamer-functionalized electrode arrays can provide an intriguing method for detecting pathogen-derived exometabolites. This work addresses the limitations of previous aptamer-based pathogen detection methods by introducing a novel surface design that bridges the gap between initial efforts in this area and the demands of a point-of-care device. Specifically, the use of a diblock copolymer coating on a high-density microelectrode array and Cu-mediated cross coupling reactions that allow for the exclusive functionalization of that coating by any electrode or set of electrodes in the array provides a device that is stable for 1 year and compatible with the multiplex detection of small-molecule targets. The new chemistry developed allows one to take advantage of a large number of electrodes in the array with one experiment described herein capitalizing on the use of 960 individually addressable electrodes.
UR - http://www.scopus.com/inward/record.url?scp=85202073692&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.4c02978
DO - 10.1021/acs.analchem.4c02978
M3 - Article
C2 - 39183484
AN - SCOPUS:85202073692
SN - 0003-2700
VL - 96
SP - 14571
EP - 14580
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 36
ER -