Determination of subsurface tumor localization in animal models with near-infrared (NIR) fluorescence imaging

Yu Chen, Dana Blessington, Zhihong Zhang, Qian Liu, Lanlan Zhou, Chenpeng Mu, Xavier Intes, Samuel Achilefu, Hui Li, Min Zhang, Gang Zheng, Britton Chance

Research output: Contribution to journalConference articlepeer-review

1 Scopus citations

Abstract

We have developed a novel imaging system for determining the localization of tumors labeled by fluorescent contrast agents and embedded several centimeters inside the highly scattering medium. This frequency-domain system utilizes the phased cancellation configuration with a goniometric probe. The instrumentation performance on the phantom test can detect 3 mm diameter sphere filled with 1 nM fluorescent dye, Indocyanine Green (ICG), and 3 cm deep inside the scattering medium with similar optical properties as human breast tissue within a 1 mm localization confidence. Mouse tumor model immersed in appropriate scattering/absorbing medium is used for animal test. Intra-tumor injection of ICG demonstrates the localization of the tumor (5 mm in diameter) submerged 3 cm deep inside the highly scattering medium with 2 mm position error. Results with NIR804-D-Glucosamide on the AR42J tumor bearing nude mouse are also presented with 3 mm localization error. The accuracy of the localization suggests that this system would be helpful to guide the clinical fine-needle biopsy for early breast cancer detection.

Original languageEnglish
Pages (from-to)322-329
Number of pages8
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume4955
DOIs
StatePublished - Dec 5 2003
EventPROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Optical Tomography and Spectroscopy of Tissue V - San Jose, CA, United States
Duration: Jan 26 2003Jan 29 2003

Keywords

  • Early tumor detection
  • Fluorescence
  • Near-infrared
  • Phased array
  • Tricarbocaynine

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