Determination of subsurface tumor localization in animal models with near-infrared (NIR) fluorescence imaging

Yu Chen; Dana Blessington; Zhihong Zhang; Qian Liu; Lanlan Zhou; Chenpeng Mu; Xavier Intes; Samuel Achilefu; Hui Li; Min Zhang; Gang Zheng; Britton Chance

doi:10.1117/12.478187

Determination of subsurface tumor localization in animal models with near-infrared (NIR) fluorescence imaging

Yu Chen, Dana Blessington, Zhihong Zhang, Qian Liu, Lanlan Zhou, Chenpeng Mu, Xavier Intes, Samuel Achilefu, Hui Li, Min Zhang, Gang Zheng, Britton Chance

Research output: Contribution to journal › Conference article › peer-review

1 Scopus citations

Abstract

We have developed a novel imaging system for determining the localization of tumors labeled by fluorescent contrast agents and embedded several centimeters inside the highly scattering medium. This frequency-domain system utilizes the phased cancellation configuration with a goniometric probe. The instrumentation performance on the phantom test can detect 3 mm diameter sphere filled with 1 nM fluorescent dye, Indocyanine Green (ICG), and 3 cm deep inside the scattering medium with similar optical properties as human breast tissue within a 1 mm localization confidence. Mouse tumor model immersed in appropriate scattering/absorbing medium is used for animal test. Intra-tumor injection of ICG demonstrates the localization of the tumor (5 mm in diameter) submerged 3 cm deep inside the highly scattering medium with 2 mm position error. Results with NIR804-D-Glucosamide on the AR42J tumor bearing nude mouse are also presented with 3 mm localization error. The accuracy of the localization suggests that this system would be helpful to guide the clinical fine-needle biopsy for early breast cancer detection.

Original language	English
Pages (from-to)	322-329
Number of pages	8
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	4955
DOIs	https://doi.org/10.1117/12.478187
State	Published - Dec 5 2003
Event	PROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Optical Tomography and Spectroscopy of Tissue V - San Jose, CA, United States Duration: Jan 26 2003 → Jan 29 2003

Keywords

Early tumor detection
Fluorescence
Near-infrared
Phased array
Tricarbocaynine

Access to Document

10.1117/12.478187

Cite this

Chen, Y., Blessington, D., Zhang, Z., Liu, Q., Zhou, L., Mu, C., Intes, X., Achilefu, S., Li, H., Zhang, M., Zheng, G., & Chance, B. (2003). Determination of subsurface tumor localization in animal models with near-infrared (NIR) fluorescence imaging. Proceedings of SPIE - The International Society for Optical Engineering, 4955, 322-329. https://doi.org/10.1117/12.478187

@article{b59919c025824f239bac6ed4fe2d10a4,

title = "Determination of subsurface tumor localization in animal models with near-infrared (NIR) fluorescence imaging",

abstract = "We have developed a novel imaging system for determining the localization of tumors labeled by fluorescent contrast agents and embedded several centimeters inside the highly scattering medium. This frequency-domain system utilizes the phased cancellation configuration with a goniometric probe. The instrumentation performance on the phantom test can detect 3 mm diameter sphere filled with 1 nM fluorescent dye, Indocyanine Green (ICG), and 3 cm deep inside the scattering medium with similar optical properties as human breast tissue within a 1 mm localization confidence. Mouse tumor model immersed in appropriate scattering/absorbing medium is used for animal test. Intra-tumor injection of ICG demonstrates the localization of the tumor (5 mm in diameter) submerged 3 cm deep inside the highly scattering medium with 2 mm position error. Results with NIR804-D-Glucosamide on the AR42J tumor bearing nude mouse are also presented with 3 mm localization error. The accuracy of the localization suggests that this system would be helpful to guide the clinical fine-needle biopsy for early breast cancer detection.",

keywords = "Early tumor detection, Fluorescence, Near-infrared, Phased array, Tricarbocaynine",

author = "Yu Chen and Dana Blessington and Zhihong Zhang and Qian Liu and Lanlan Zhou and Chenpeng Mu and Xavier Intes and Samuel Achilefu and Hui Li and Min Zhang and Gang Zheng and Britton Chance",

year = "2003",

month = dec,

day = "5",

doi = "10.1117/12.478187",

language = "English",

volume = "4955",

pages = "322--329",

journal = "Proceedings of SPIE - The International Society for Optical Engineering",

issn = "0277-786X",

note = "PROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Optical Tomography and Spectroscopy of Tissue V ; Conference date: 26-01-2003 Through 29-01-2003",

}

TY - JOUR

T1 - Determination of subsurface tumor localization in animal models with near-infrared (NIR) fluorescence imaging

AU - Chen, Yu

AU - Blessington, Dana

AU - Zhang, Zhihong

AU - Liu, Qian

AU - Zhou, Lanlan

AU - Mu, Chenpeng

AU - Intes, Xavier

AU - Achilefu, Samuel

AU - Li, Hui

AU - Zhang, Min

AU - Zheng, Gang

AU - Chance, Britton

PY - 2003/12/5

Y1 - 2003/12/5

N2 - We have developed a novel imaging system for determining the localization of tumors labeled by fluorescent contrast agents and embedded several centimeters inside the highly scattering medium. This frequency-domain system utilizes the phased cancellation configuration with a goniometric probe. The instrumentation performance on the phantom test can detect 3 mm diameter sphere filled with 1 nM fluorescent dye, Indocyanine Green (ICG), and 3 cm deep inside the scattering medium with similar optical properties as human breast tissue within a 1 mm localization confidence. Mouse tumor model immersed in appropriate scattering/absorbing medium is used for animal test. Intra-tumor injection of ICG demonstrates the localization of the tumor (5 mm in diameter) submerged 3 cm deep inside the highly scattering medium with 2 mm position error. Results with NIR804-D-Glucosamide on the AR42J tumor bearing nude mouse are also presented with 3 mm localization error. The accuracy of the localization suggests that this system would be helpful to guide the clinical fine-needle biopsy for early breast cancer detection.

AB - We have developed a novel imaging system for determining the localization of tumors labeled by fluorescent contrast agents and embedded several centimeters inside the highly scattering medium. This frequency-domain system utilizes the phased cancellation configuration with a goniometric probe. The instrumentation performance on the phantom test can detect 3 mm diameter sphere filled with 1 nM fluorescent dye, Indocyanine Green (ICG), and 3 cm deep inside the scattering medium with similar optical properties as human breast tissue within a 1 mm localization confidence. Mouse tumor model immersed in appropriate scattering/absorbing medium is used for animal test. Intra-tumor injection of ICG demonstrates the localization of the tumor (5 mm in diameter) submerged 3 cm deep inside the highly scattering medium with 2 mm position error. Results with NIR804-D-Glucosamide on the AR42J tumor bearing nude mouse are also presented with 3 mm localization error. The accuracy of the localization suggests that this system would be helpful to guide the clinical fine-needle biopsy for early breast cancer detection.

KW - Early tumor detection

KW - Fluorescence

KW - Near-infrared

KW - Phased array

KW - Tricarbocaynine

UR - http://www.scopus.com/inward/record.url?scp=0345714901&partnerID=8YFLogxK

U2 - 10.1117/12.478187

DO - 10.1117/12.478187

M3 - Conference article

AN - SCOPUS:0345714901

SN - 0277-786X

VL - 4955

SP - 322

EP - 329

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - PROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Optical Tomography and Spectroscopy of Tissue V

Y2 - 26 January 2003 through 29 January 2003

ER -

Determination of subsurface tumor localization in animal models with near-infrared (NIR) fluorescence imaging

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this