TY - JOUR
T1 - Determination of benzidine, N-acetylbenzidine, and N,N′-diacetylbenzidine in human urine by capillary gas chromatography/negative ion chemical ionization mass spectrometry
AU - Hsu, F. F.
AU - Lakshmi, V.
AU - Rothman, N.
AU - Bhatnager, V. K.
AU - Hayes, R. B.
AU - Kashyap, R.
AU - Parikh, D. J.
AU - Kashyap, S. K.
AU - Turk, J.
AU - Zenser, T.
AU - Davis, B.
N1 - Funding Information:
This work was supported by the Department of Veterans Affairs (T.V.Z., B.B.D.) and American Cancer Society Grant CN-69B (T.V.Z.). Mass spectrometry was performed at Center of Mass Spectrometry Resource at Washington University School of Medicine (St. Louis, MO) through NIH Grants RR-00954 and AM-20579. The authors thank Priscilla DeHaven for excellent technical assistance.
PY - 1996/2/15
Y1 - 1996/2/15
N2 - We report an isotope dilution mass spectrometry method, using capillary gas chromatography/negative ion chemical ionization to quantitate urine concentrations of benzidine (BZ) and of its acetylated metabolites N-acetylbenzidine (ABZ) and N,N′-diacetylbenzidine (DABZ). Urine samples were purified by solid-phase extraction columns, reduced with LiAlH4/ THF, and derivatized with pentafluoropropionic anhydride. The derivatives were measured by selected ion monitoring relative to deuterium-labeled internal standards. A detection limit as low as 0.5, 0.8, and 1.5 ppt for BZ, ABZ, and DABZ, respectively, can easily be achieved. Urinary concentrations of ABZ substantially exceed those of either BZ or of DABZ in workers ex-posed to BZ or BZ-based dyes. This method has been successfully used to measure BZ, ABZ, and DABZ in 1.0-ml urine samples collected from workers involved in manufacturing BZ and BZ-based dyes. The method should be applicable to the measurement of other aromatic amines and their acetylated metabolites.
AB - We report an isotope dilution mass spectrometry method, using capillary gas chromatography/negative ion chemical ionization to quantitate urine concentrations of benzidine (BZ) and of its acetylated metabolites N-acetylbenzidine (ABZ) and N,N′-diacetylbenzidine (DABZ). Urine samples were purified by solid-phase extraction columns, reduced with LiAlH4/ THF, and derivatized with pentafluoropropionic anhydride. The derivatives were measured by selected ion monitoring relative to deuterium-labeled internal standards. A detection limit as low as 0.5, 0.8, and 1.5 ppt for BZ, ABZ, and DABZ, respectively, can easily be achieved. Urinary concentrations of ABZ substantially exceed those of either BZ or of DABZ in workers ex-posed to BZ or BZ-based dyes. This method has been successfully used to measure BZ, ABZ, and DABZ in 1.0-ml urine samples collected from workers involved in manufacturing BZ and BZ-based dyes. The method should be applicable to the measurement of other aromatic amines and their acetylated metabolites.
UR - http://www.scopus.com/inward/record.url?scp=0030063452&partnerID=8YFLogxK
U2 - 10.1006/abio.1996.0070
DO - 10.1006/abio.1996.0070
M3 - Article
C2 - 8714596
AN - SCOPUS:0030063452
SN - 0003-2697
VL - 234
SP - 183
EP - 189
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -