The determination of peptide extract taken from l-A g7 cell line was discussed. The peptides were extracted from l-A g7 and other structurally similar MHC (l-A g7pd and l-A d) and peptide repertoire of homozygotic cell lines were compared to that of heterozygotic cell lines. A large number of peptides were analyzed using tendem MS. 25 peptide sequences were determined using MASCOT as the database search program, by using reverse phase-nano-LC and the LCQ-deca. The results show that most of the peptides selected by l-A g7 are unusually rich in acidic residues toward their C-termini.
|Number of pages||2|
|State||Published - Dec 1 2002|
|Event||Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States|
Duration: Jun 2 2002 → Jun 6 2002
|Conference||Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics|
|Period||06/2/02 → 06/6/02|