Determination of amino acid isotopic enrichment: Methods, difficulties, and calculations

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Stable isotopically labeled amino acids are used extensively in studies of protein and amino acid metabolism at the whole-body and tissue levels. The gas chromatograph-mass spectrometer (GC-MS) is a versatile instrument to measure the isotopic enrichment of amino acids. Investigators today are faced with diverse GC-MS methods, which combine a variety of derivatives available for amino acid separation by gas chromatography (GC) with different ionization modes for mass spectrometer (MS) operation. Adding to this diversity is a variety of approaches that are used to calculate isotopic enrichments from GC-MS measurements.

Original languageEnglish
Title of host publicationMethods for Investigation of Amino Acid and Protein Metabolism
PublisherCRC Press
Pages103-119
Number of pages17
ISBN (Electronic)9781351431934
ISBN (Print)9780849396120
DOIs
StatePublished - Jan 1 2017

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