Determinants of directionality in lambda site-specific recombination

Wade Bushman; Samuel Yin; Liu Lin Thio; Arthur Landy

doi:10.1016/0092-8674(84)90477-X

Determinants of directionality in lambda site-specific recombination

Wade Bushman, Samuel Yin, Liu Lin Thio, Arthur Landy

Research output: Contribution to journal › Article › peer-review

69 Scopus citations

Abstract

The DNA structural features governing directionality in λ site-specific recombination are shown to reside in regions of the phage attachment site more than 70 bp to the left and more than 40 bp to the right of the cross-over region. Disposition of these sequences on the same attachment site in integration, and on different attachment sites in excision, determines the opposite effects of Xis protein upon the two reactions (stimulation of excision and inhibition of integration). The binding of Xis to two adjacent directly repeated sequences in the left phage arm is shown to occur in a highly cooperative manner, to alter the conformation of the DNA, and to produce a 32-fold stimulation of Int binding to an adjacent locus.

Original language	English
Pages (from-to)	699-706
Number of pages	8
Journal	Cell
Volume	39
Issue number	3 PART 2
DOIs	https://doi.org/10.1016/0092-8674(84)90477-X
State	Published - Dec 1984

Access to Document

10.1016/0092-8674(84)90477-X

Cite this

@article{7e346923b94e435588f0b091750207a1,

title = "Determinants of directionality in lambda site-specific recombination",

abstract = "The DNA structural features governing directionality in λ site-specific recombination are shown to reside in regions of the phage attachment site more than 70 bp to the left and more than 40 bp to the right of the cross-over region. Disposition of these sequences on the same attachment site in integration, and on different attachment sites in excision, determines the opposite effects of Xis protein upon the two reactions (stimulation of excision and inhibition of integration). The binding of Xis to two adjacent directly repeated sequences in the left phage arm is shown to occur in a highly cooperative manner, to alter the conformation of the DNA, and to produce a 32-fold stimulation of Int binding to an adjacent locus.",

author = "Wade Bushman and Samuel Yin and Thio, {Liu Lin} and Arthur Landy",

note = "Funding Information: We thank Ken Abremskt and Sue Wickner for advlce on Xis purlflcatlon, Klyoshl Mlzuuchl for plasmid pMM291; David Brautlgan, Sungman Cha, and Mike Fried for helpful dlscussions; Llna Vargas for assistance with DNA sequencing and nuclease-protectjon experiments and Shirley Rodrlgues for technlcai assistance. This work was supported by grant Al 13544 from the National lnstltutes of Health. S. Y. was a recipient of a Damon Runyon - Walter Winchell Cancer Fund postdoctoral fellowshlp.",

year = "1984",

month = dec,

doi = "10.1016/0092-8674(84)90477-X",

language = "English",

volume = "39",

pages = "699--706",

journal = "Cell",

issn = "0092-8674",

number = "3 PART 2",

}

TY - JOUR

T1 - Determinants of directionality in lambda site-specific recombination

AU - Bushman, Wade

AU - Yin, Samuel

AU - Thio, Liu Lin

AU - Landy, Arthur

N1 - Funding Information: We thank Ken Abremskt and Sue Wickner for advlce on Xis purlflcatlon, Klyoshl Mlzuuchl for plasmid pMM291; David Brautlgan, Sungman Cha, and Mike Fried for helpful dlscussions; Llna Vargas for assistance with DNA sequencing and nuclease-protectjon experiments and Shirley Rodrlgues for technlcai assistance. This work was supported by grant Al 13544 from the National lnstltutes of Health. S. Y. was a recipient of a Damon Runyon - Walter Winchell Cancer Fund postdoctoral fellowshlp.

PY - 1984/12

Y1 - 1984/12

N2 - The DNA structural features governing directionality in λ site-specific recombination are shown to reside in regions of the phage attachment site more than 70 bp to the left and more than 40 bp to the right of the cross-over region. Disposition of these sequences on the same attachment site in integration, and on different attachment sites in excision, determines the opposite effects of Xis protein upon the two reactions (stimulation of excision and inhibition of integration). The binding of Xis to two adjacent directly repeated sequences in the left phage arm is shown to occur in a highly cooperative manner, to alter the conformation of the DNA, and to produce a 32-fold stimulation of Int binding to an adjacent locus.

AB - The DNA structural features governing directionality in λ site-specific recombination are shown to reside in regions of the phage attachment site more than 70 bp to the left and more than 40 bp to the right of the cross-over region. Disposition of these sequences on the same attachment site in integration, and on different attachment sites in excision, determines the opposite effects of Xis protein upon the two reactions (stimulation of excision and inhibition of integration). The binding of Xis to two adjacent directly repeated sequences in the left phage arm is shown to occur in a highly cooperative manner, to alter the conformation of the DNA, and to produce a 32-fold stimulation of Int binding to an adjacent locus.

UR - http://www.scopus.com/inward/record.url?scp=0021676510&partnerID=8YFLogxK

U2 - 10.1016/0092-8674(84)90477-X

DO - 10.1016/0092-8674(84)90477-X

M3 - Article

C2 - 6239693

AN - SCOPUS:0021676510

SN - 0092-8674

VL - 39

SP - 699

EP - 706

JO - Cell

JF - Cell

IS - 3 PART 2

ER -

Determinants of directionality in lambda site-specific recombination

Abstract

Access to Document

Other files and links

Fingerprint

Cite this