Detection of putative secreted proteins in the plant-parasitic nematode Heterodera schachtii

Bartel Vanholme, Makedonka Mitreva, Wim Van Criekinge, Marc Logghe, David Bird, James P. McCarter, Godelieve Gheysen

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

The beet cyst nematode Heterodera schachtii is an important pathogen worldwide, but its molecular characterization has been limited to studying individual genes of interest. We undertook a high-throughput genomic approach and drastically increased the number of available sequences for this parasite. A total of 2,662 expressed sequence tags were grouped into 1,212 clusters representing a nonredundant catalog of H. schachtii genes. Implementing a bioinformatic workflow, we identified 50 sequences coding for candidate secreted proteins. All of these contain a putative signal peptide required for entry into the secretory pathway and lack any transmembrane domain. Included are previously postulated cell-wall-degrading enzymes and other parasitism-related genes. Moreover, we provide the first report of an arabinogalactan endo-1,4-β-galactosidase enzyme (EC 3.2.1.89) in animals. As sequence data increase at a rapid rate, developing high-throughput genomic screening is a necessity. The in silico approach described here is an effective way to identify putative secreted proteins and prioritize candidates for further studies.

Original languageEnglish
Pages (from-to)414-424
Number of pages11
JournalParasitology Research
Volume98
Issue number5
DOIs
StatePublished - Apr 2006

Fingerprint

Dive into the research topics of 'Detection of putative secreted proteins in the plant-parasitic nematode Heterodera schachtii'. Together they form a unique fingerprint.

Cite this