Detection of murine norovirus 1 by using plaque assay, transfection assay, and real-time reverse transcription-PCR before and after heat exposure

Leen Baert; Christiane E. Wobus; Els Van Coillie; Larissa B. Thackray; Johan Debevere; Mieke Uyttendaele

doi:10.1128/AEM.01039-07

Detection of murine norovirus 1 by using plaque assay, transfection assay, and real-time reverse transcription-PCR before and after heat exposure

Leen Baert
, Christiane E. Wobus
, Els Van Coillie
, Larissa B. Thackray
, Johan Debevere
, Mieke Uyttendaele

Division of Infectious Diseases

Research output: Contribution to journal › Article › peer-review

248 Scopus citations

Abstract

The correlation between the detection of murine norovirus 1 RNA by real-time reverse transcription-PCR and the infectivity by plaque assay before and after heat exposure (80°C) was examined. No correlation was found in the current study. Moreover, heat inactivation had a much stronger detrimental effect on virus infectivity than on the integrity of the viral genome.

Original language	English
Pages (from-to)	543-546
Number of pages	4
Journal	Applied and Environmental Microbiology
Volume	74
Issue number	2
DOIs	https://doi.org/10.1128/AEM.01039-07
State	Published - Jan 2008

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title = "Detection of murine norovirus 1 by using plaque assay, transfection assay, and real-time reverse transcription-PCR before and after heat exposure",

abstract = "The correlation between the detection of murine norovirus 1 RNA by real-time reverse transcription-PCR and the infectivity by plaque assay before and after heat exposure (80°C) was examined. No correlation was found in the current study. Moreover, heat inactivation had a much stronger detrimental effect on virus infectivity than on the integrity of the viral genome.",

author = "Leen Baert and Wobus, \{Christiane E.\} and \{Van Coillie\}, Els and Thackray, \{Larissa B.\} and Johan Debevere and Mieke Uyttendaele",

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AU - Baert, Leen

AU - Wobus, Christiane E.

AU - Van Coillie, Els

AU - Thackray, Larissa B.

AU - Debevere, Johan

AU - Uyttendaele, Mieke

PY - 2008/1

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AB - The correlation between the detection of murine norovirus 1 RNA by real-time reverse transcription-PCR and the infectivity by plaque assay before and after heat exposure (80°C) was examined. No correlation was found in the current study. Moreover, heat inactivation had a much stronger detrimental effect on virus infectivity than on the integrity of the viral genome.

UR - https://www.scopus.com/pages/publications/38349194050

U2 - 10.1128/AEM.01039-07

DO - 10.1128/AEM.01039-07

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JO - Applied and Environmental Microbiology

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ER -

Detection of murine norovirus 1 by using plaque assay, transfection assay, and real-time reverse transcription-PCR before and after heat exposure

Abstract

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