TY - JOUR
T1 - Detection of murine norovirus 1 by using plaque assay, transfection assay, and real-time reverse transcription-PCR before and after heat exposure
AU - Baert, Leen
AU - Wobus, Christiane E.
AU - Van Coillie, Els
AU - Thackray, Larissa B.
AU - Debevere, Johan
AU - Uyttendaele, Mieke
PY - 2008/1
Y1 - 2008/1
N2 - The correlation between the detection of murine norovirus 1 RNA by real-time reverse transcription-PCR and the infectivity by plaque assay before and after heat exposure (80°C) was examined. No correlation was found in the current study. Moreover, heat inactivation had a much stronger detrimental effect on virus infectivity than on the integrity of the viral genome.
AB - The correlation between the detection of murine norovirus 1 RNA by real-time reverse transcription-PCR and the infectivity by plaque assay before and after heat exposure (80°C) was examined. No correlation was found in the current study. Moreover, heat inactivation had a much stronger detrimental effect on virus infectivity than on the integrity of the viral genome.
UR - http://www.scopus.com/inward/record.url?scp=38349194050&partnerID=8YFLogxK
U2 - 10.1128/AEM.01039-07
DO - 10.1128/AEM.01039-07
M3 - Article
C2 - 18024676
AN - SCOPUS:38349194050
SN - 0099-2240
VL - 74
SP - 543
EP - 546
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
IS - 2
ER -