Detection of murine norovirus 1 by using plaque assay, transfection assay, and real-time reverse transcription-PCR before and after heat exposure

Leen Baert, Christiane E. Wobus, Els Van Coillie, Larissa B. Thackray, Johan Debevere, Mieke Uyttendaele

Research output: Contribution to journalArticlepeer-review

237 Scopus citations

Abstract

The correlation between the detection of murine norovirus 1 RNA by real-time reverse transcription-PCR and the infectivity by plaque assay before and after heat exposure (80°C) was examined. No correlation was found in the current study. Moreover, heat inactivation had a much stronger detrimental effect on virus infectivity than on the integrity of the viral genome.

Original languageEnglish
Pages (from-to)543-546
Number of pages4
JournalApplied and Environmental Microbiology
Volume74
Issue number2
DOIs
StatePublished - Jan 2008

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