TY - JOUR
T1 - Detection of Loxosceles venom in lesional hair shafts and skin
T2 - Application of a specific immunoassay to identify dermonecrotic arachnidism
AU - Miller, Mark J.
AU - Gomez, Hernan F.
AU - Snider, Richard J.
AU - Stephens, Edward L.
AU - Czop, Richard M.
AU - Warren, Jeffrey S.
PY - 2000/1/1
Y1 - 2000/1/1
N2 - Loxosceles spiders, of which the brown recluse is the best known, are indigenous to southcentral and southwestern regions of the United States. Loxosceles spider envenomation frequently results in painful, centrally necrotic, erythematous skin lesions that evolve over 24 to 48 hours and may take several weeks to completely heal. The diagnosis of loxoscelism is typically is based on the presence of the characteristic dermal lesion, because no definitive clinical diagnostic assay exists, and the spider is generally not available for identification. We used a rapid Loxosceles-specific enzyme immunoassay to detect spider venom in a dermal biopsy and hairs plucked from a suspicious skin lesion on the lower extremity of a 52-year-old man. This report indicates that in using a novel Loxosceles-specific immunoassay, venom can be detected in dermonecrotic skin and hair specimens for up to 4 days after envenomation. Copyright (C) 2000 by W.B. Saunders Company.
AB - Loxosceles spiders, of which the brown recluse is the best known, are indigenous to southcentral and southwestern regions of the United States. Loxosceles spider envenomation frequently results in painful, centrally necrotic, erythematous skin lesions that evolve over 24 to 48 hours and may take several weeks to completely heal. The diagnosis of loxoscelism is typically is based on the presence of the characteristic dermal lesion, because no definitive clinical diagnostic assay exists, and the spider is generally not available for identification. We used a rapid Loxosceles-specific enzyme immunoassay to detect spider venom in a dermal biopsy and hairs plucked from a suspicious skin lesion on the lower extremity of a 52-year-old man. This report indicates that in using a novel Loxosceles-specific immunoassay, venom can be detected in dermonecrotic skin and hair specimens for up to 4 days after envenomation. Copyright (C) 2000 by W.B. Saunders Company.
KW - Brown recluse and spider venom enzyme immunoassay
KW - Dermonecrotic arachnidism
KW - Loxosceles
UR - http://www.scopus.com/inward/record.url?scp=0033827580&partnerID=8YFLogxK
U2 - 10.1053/ajem.2000.9272
DO - 10.1053/ajem.2000.9272
M3 - Article
C2 - 10999583
AN - SCOPUS:0033827580
VL - 18
SP - 626
EP - 628
JO - American Journal of Emergency Medicine
JF - American Journal of Emergency Medicine
SN - 0735-6757
IS - 5
ER -