Detection of HTLV-I in peripheral blood lymphocytes from patients with chronic HTLV-I-associated myelopathy/tropical spastic paraparesis and asymptomatic carriers by PCR-in situ hybridization

Matthew J. Walter, Tanya J. Lehky, Michael C. Levin, Cecil H. Fox, Steven Jacobson

Research output: Contribution to journalArticle

Abstract

Less than 5% of people infected with human T-lymphotropic virus type I HTLV-I-associated (HTLV-I) develop HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP), a chronic progressive neurologic disease. A number of factors have been implicated in the development of HAM/TSP including heterogeneity of viral sequences, host-genetic background, viral-specific cellular immune responses and viral load. This study examined the presence of HTLV-I tax DNA in peripheral blood lymphocytes (PBL) from 2 chronic HAM/TSP patients and 2 asymptomatic HTLV-I carriers by using PCR-in situ hybridization (PCR-ISH) for the in situ presence of proviral HTLV-I tax DNA. By this technique, rare PBL from these HTLV-I-infected individuals contained HTLV-I DNA. PCR-ISH did not detect any difference in the number of infected cells between HAM/TSP patients and asymptomatic carriers.

Original languageEnglish
Pages (from-to)54-60
Number of pages7
JournalJournal of Biomedical Science
Volume4
Issue number1
DOIs
StatePublished - Jan 1 1997
Externally publishedYes

Keywords

  • HTLV-I infection
  • HTLV-I-associated myelopathy/tropical spastic paraparesis
  • PCR-in situ hybridization

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