Detection of biomarkers for filoviral infection with a silicon photonic resonator platform

Krista Meserve, Abraham J. Qavi, M. Javad Aman, Hong Vu, Larry Zeitlin, John M. Dye, Jeffrey W. Froude, Daisy W. Leung, Lan Yang, Frederick W. Holtsberg, Gaya K. Amarasinghe, Ryan C. Bailey

Research output: Contribution to journalArticlepeer-review

Abstract

This protocol describes the use of silicon photonic microring resonator sensors for detection of Ebola virus (EBOV) and Sudan virus (SUDV) soluble glycoprotein (sGP). This protocol encompasses biosensor functionalization of silicon microring resonator chips, detection of protein biomarkers in sera, preparing calibration standards for analytical validation, and quantification of the results from these experiments. This protocol is readily adaptable toward other analytes, including cytokines, chemokines, nucleic acids, and viruses. For complete details on the use and execution of this protocol, please refer to Qavi et al. (2022).

Original languageEnglish
Article number101719
JournalSTAR Protocols
Volume3
Issue number4
DOIs
StatePublished - Dec 16 2022

Keywords

  • Antibody
  • Biotechnology and bioengineering
  • Chemistry
  • Health sciences

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