TY - JOUR
T1 - Detection and measurement of neurofibromatosis-1 mouse optic glioma in vivo
AU - Banerjee, Debasish
AU - Hegedus, Balazs
AU - Gutmann, David H.
AU - Garbow, Joel R.
N1 - Funding Information:
This work was supported by an NIH/NCI Small Animal Imaging Resource Program (SAIRP) grant (R24 CA83060); Department of Defense grant W81XWH061022; NIH grant NS054629-01; and the Alvin J. Siteman Cancer Center at Washington University in St. Louis, an NCI Comprehensive Cancer Center (P30 CA91842).
PY - 2007/5/1
Y1 - 2007/5/1
N2 - One of the major limitations to preclinical mouse therapeutic evaluation is the inherent difficulty in imaging small tumors in vivo. We present a rapid and reliable method to detect optic glioma (OPG) in a mouse neurofibromatosis-1 model (Nf1flox/mutGFAP-Cre mice) in vivo using Manganese-Enhanced Magnetic Resonance Imaging (MEMRI). In a blinded study, 23 mice were chosen randomly from a cohort of Nf1flox/mutGFAP-Cre mice and two sets of age-matched controls. In all cases, OPG presence or absence was correctly identified. In addition, the OPG size and shape were accurately measured in vivo, facilitating the use of this model for preclinical drug studies.
AB - One of the major limitations to preclinical mouse therapeutic evaluation is the inherent difficulty in imaging small tumors in vivo. We present a rapid and reliable method to detect optic glioma (OPG) in a mouse neurofibromatosis-1 model (Nf1flox/mutGFAP-Cre mice) in vivo using Manganese-Enhanced Magnetic Resonance Imaging (MEMRI). In a blinded study, 23 mice were chosen randomly from a cohort of Nf1flox/mutGFAP-Cre mice and two sets of age-matched controls. In all cases, OPG presence or absence was correctly identified. In addition, the OPG size and shape were accurately measured in vivo, facilitating the use of this model for preclinical drug studies.
UR - http://www.scopus.com/inward/record.url?scp=34147113875&partnerID=8YFLogxK
U2 - 10.1016/j.neuroimage.2007.02.019
DO - 10.1016/j.neuroimage.2007.02.019
M3 - Article
C2 - 17383899
AN - SCOPUS:34147113875
SN - 1053-8119
VL - 35
SP - 1434
EP - 1437
JO - NeuroImage
JF - NeuroImage
IS - 4
ER -