TY - JOUR
T1 - Desorption ionization combined with tandem mass spectrometry
T2 - Advantages for investigating complex lipids, disaccharides and organometallic complexes
AU - Cerny, Ronald L.
AU - Tomer, Kenneth B.
AU - Gross, Michael L.
PY - 1986/10
Y1 - 1986/10
N2 - Combining desorption ionization with tandem mass spectrometry overcomes the disadvantage of limited fragmentation accompanying desorption and permits mixtures of closely related substances to be investigated directly. These features of the combination are illustrated by completing the structure‐proof of a minor component of an ornithine‐containing lipid mixture isolated from Thiobacillus thiooxidans. The minor component is a homolog of the major constituent and differs from the principal component owing to the presence of a double bond in lieu of a cyclopropyl ring in one of the constituent fatty acids. Another feature of the combined method is the potentially complementary nature of collision‐activated dissociation spectra of protonated and cationized biomolecules. This is illustrated by the differences in the collision‐activated dissociations of the [M + Na]+ of sucrose, desorbed by field desorption, and [M + H]+, desorbed by fast atom bombardment. A third illustration is the application of field desorption and tandem mass spectrometry to an organometallic compound. The combined approach allows the ligands to be identified and the relative ligand binding energies to be approximated.
AB - Combining desorption ionization with tandem mass spectrometry overcomes the disadvantage of limited fragmentation accompanying desorption and permits mixtures of closely related substances to be investigated directly. These features of the combination are illustrated by completing the structure‐proof of a minor component of an ornithine‐containing lipid mixture isolated from Thiobacillus thiooxidans. The minor component is a homolog of the major constituent and differs from the principal component owing to the presence of a double bond in lieu of a cyclopropyl ring in one of the constituent fatty acids. Another feature of the combined method is the potentially complementary nature of collision‐activated dissociation spectra of protonated and cationized biomolecules. This is illustrated by the differences in the collision‐activated dissociations of the [M + Na]+ of sucrose, desorbed by field desorption, and [M + H]+, desorbed by fast atom bombardment. A third illustration is the application of field desorption and tandem mass spectrometry to an organometallic compound. The combined approach allows the ligands to be identified and the relative ligand binding energies to be approximated.
UR - http://www.scopus.com/inward/record.url?scp=84989092818&partnerID=8YFLogxK
U2 - 10.1002/oms.1210211009
DO - 10.1002/oms.1210211009
M3 - Article
AN - SCOPUS:84989092818
SN - 0030-493X
VL - 21
SP - 655
EP - 660
JO - Organic Mass Spectrometry
JF - Organic Mass Spectrometry
IS - 10
ER -