Designing large field-of-view two photon microscopy using optical invariant analysis and off-the-shelf components

Jonathan R. Bumstead; Isaac A. Rosen; Andrew W. Kraft; Patrick W. Wright; Daniel Coˆte´; Joseph P. Culver

doi:10.1364/BRAIN.2017.BrM4B.2

Designing large field-of-view two photon microscopy using optical invariant analysis and off-the-shelf components

Jonathan R. Bumstead, Isaac A. Rosen, Andrew W. Kraft, Patrick W. Wright, Daniel Coˆte´, Joseph P. Culver

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Conventional two photon microscopy (TPM) is capable of imaging neural dynamics with subcellular resolution, but is limited to a field of view (FOV) >Ø1mm. We present a strategy for extending the FOV to Ø8mm while maintaining cellular resolution.

Original language	English
Title of host publication	Optics and the Brain, BRAIN 2017
Publisher	OSA - The Optical Society
ISBN (Electronic)	9781557528209
DOIs	https://doi.org/10.1364/BRAIN.2017.BrM4B.2
State	Published - Jan 1 2017
Event	Optics and the Brain, BRAIN 2017 - San Diego, United States Duration: Apr 2 2017 → Apr 5 2017

Publication series

Name	Optics InfoBase Conference Papers
Volume	Part F76-BRAIN 2017

Conference

Conference	Optics and the Brain, BRAIN 2017
Country	United States
City	San Diego
Period	04/2/17 → 04/5/17

Access to Document

10.1364/BRAIN.2017.BrM4B.2

Link to publication in Scopus

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Bumstead, J. R., Rosen, I. A., Kraft, A. W., Wright, P. W., Coˆte´, D., & Culver, J. P. (2017). Designing large field-of-view two photon microscopy using optical invariant analysis and off-the-shelf components. In Optics and the Brain, BRAIN 2017 (Optics InfoBase Conference Papers; Vol. Part F76-BRAIN 2017). OSA - The Optical Society. https://doi.org/10.1364/BRAIN.2017.BrM4B.2

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title = "Designing large field-of-view two photon microscopy using optical invariant analysis and off-the-shelf components",

abstract = "Conventional two photon microscopy (TPM) is capable of imaging neural dynamics with subcellular resolution, but is limited to a field of view (FOV) >{\O}1mm. We present a strategy for extending the FOV to {\O}8mm while maintaining cellular resolution.",

author = "Bumstead, {Jonathan R.} and Rosen, {Isaac A.} and Kraft, {Andrew W.} and Wright, {Patrick W.} and Daniel Coˆte´ and Culver, {Joseph P.}",

year = "2017",

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day = "1",

doi = "10.1364/BRAIN.2017.BrM4B.2",

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series = "Optics InfoBase Conference Papers",

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booktitle = "Optics and the Brain, BRAIN 2017",

note = "null ; Conference date: 02-04-2017 Through 05-04-2017",

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Bumstead, JR, Rosen, IA, Kraft, AW, Wright, PW, Coˆte´, D & Culver, JP 2017, Designing large field-of-view two photon microscopy using optical invariant analysis and off-the-shelf components. in Optics and the Brain, BRAIN 2017. Optics InfoBase Conference Papers, vol. Part F76-BRAIN 2017, OSA - The Optical Society, Optics and the Brain, BRAIN 2017, San Diego, United States, 04/2/17. https://doi.org/10.1364/BRAIN.2017.BrM4B.2

Designing large field-of-view two photon microscopy using optical invariant analysis and off-the-shelf components. / Bumstead, Jonathan R.; Rosen, Isaac A.; Kraft, Andrew W.; Wright, Patrick W.; Coˆte´, Daniel; Culver, Joseph P.

Optics and the Brain, BRAIN 2017. OSA - The Optical Society, 2017. (Optics InfoBase Conference Papers; Vol. Part F76-BRAIN 2017).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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T1 - Designing large field-of-view two photon microscopy using optical invariant analysis and off-the-shelf components

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AU - Rosen, Isaac A.

AU - Kraft, Andrew W.

AU - Wright, Patrick W.

AU - Coˆte´, Daniel

AU - Culver, Joseph P.

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AB - Conventional two photon microscopy (TPM) is capable of imaging neural dynamics with subcellular resolution, but is limited to a field of view (FOV) >Ø1mm. We present a strategy for extending the FOV to Ø8mm while maintaining cellular resolution.

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