Design, synthesis and evaluation of near infrared fluorescent pH indicators at physiological range

Zongren Zhang, Duanwen Shen, Kexian Liang, Samuel Achilefu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Biochemical processes frequently involve protonation and deprotonation, resulting in pH changes that can be monitored with pH indicators. In heterogenous media such as tissue where indicator concentration or visual observation is limited, highly sensitive pH indicators with reduced tissue autofluorescence are needed. Because of the reduced tissue autofluorescence in the near infrared (NIR) region, NIR fluorescence dyes such as indocyanine green (ICG) and its derivatives have been used to image molecular processes. Removing one of the N-subsitituent of ICG led to pH-sensitive dyes operating in the NIR region and at physiologically relevant range. Further modification of the compound and synthetic procedure allowed their conjugation to peptides for specific delivery to target cells and tissues. Synthetic strategy and preliminary results on the spectral and biological properties of such dyes and their bioconjugates are described.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
DOIs
StatePublished - May 8 2006
EventOptical Molecular Probes for Biomedical Applications - San Jose, CA, United States
Duration: Jan 22 2006Jan 24 2006

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6097
ISSN (Print)1605-7422

Conference

ConferenceOptical Molecular Probes for Biomedical Applications
CountryUnited States
CitySan Jose, CA
Period01/22/0601/24/06

Keywords

  • Acidic tumor
  • Fluorescent dye
  • Indocyanine
  • MIR
  • RGD
  • Synthesis
  • U87MG cells and optical image
  • pH sensitive

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  • Cite this

    Zhang, Z., Shen, D., Liang, K., & Achilefu, S. (2006). Design, synthesis and evaluation of near infrared fluorescent pH indicators at physiological range. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE [60970A] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6097). https://doi.org/10.1117/12.647852