Design of endonuclease restriction sites into primers for PCR cloning

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Summary: I present a software system PCRCLNG that facilitates the design of endonuclease restriction sites into the 5′-end of PCR primers. The product amplified using these primers can be directly cloned into vectors. The program estimates the annealing temperature for each primer and selects the primer pairs with comparable annealing temperature. Finally the software determines whether the PCR product can be cloned into the vector to generate in-frame gene fusion.

Original languageEnglish
Pages (from-to)1690-1691
Number of pages2
JournalBioinformatics
Volume18
Issue number12
DOIs
StatePublished - Dec 1 2002
Externally publishedYes

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