Design and analysis of ABRF-95SEQ, a recombinant protein with sequence heterogeneity

Karen S. De Jongh; Joseph Fernandez; Jay E. Gambee; Gregory A. Grant; Barbara Merrill; Kathryn L. Stone; John Rush

doi:10.1016/S1080-8914(96)80039-1

Design and analysis of ABRF-95SEQ, a recombinant protein with sequence heterogeneity

Karen S. De Jongh, Joseph Fernandez, Jay E. Gambee, Gregory A. Grant, Barbara Merrill, Kathryn L. Stone, John Rush

Research output: Contribution to journal › Article › peer-review

Original language	English
Pages (from-to)	347-358
Number of pages	12
Journal	Techniques in Protein Chemistry
Volume	7
Issue number	C
DOIs	https://doi.org/10.1016/S1080-8914(96)80039-1
State	Published - 1996

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@article{0bb559e2529143eb89f43aaa9da23a15,

title = "Design and analysis of ABRF-95SEQ, a recombinant protein with sequence heterogeneity",

author = "{De Jongh}, {Karen S.} and Joseph Fernandez and Gambee, {Jay E.} and Grant, {Gregory A.} and Barbara Merrill and Stone, {Kathryn L.} and John Rush",

note = "Funding Information: This work was partially supported by NSF grant DIR9003100 to John Crabb (W. Alton Jones Cell Science Center) on behalf of the ABRF. The contribution of all respondents that made this study possible by analyzing ABRF-95SEQ is gratefully acknowledged. The assistance of Gary P. Gryan (HHMI/Harvard Medical School Computer Facility) in coordinating the data returns and ensuring the anonymity of the participating laboratories is appreciated. The assistance of the ABRF Business Office for postcard printing and mailings is acknowledged. We thank Dr Hong Chen and Dr Geoffrey Duyk for generously providing the pGEX/ocp II expression plasmid. Several members of the authors' laboratories contributed to this project including Dawn Fitzpatrick and Cara Ruble (HHMI/Harvard Medical School) for construction of ocp variants; Ivar Jensen (HHMI/Harvard Medical School) for protein sequence analysis of ocp variants; Jeffry Bondor (Shriners Hospital for Crippled Children) for sample distribution and Bill Chestnut (Burroughs Wellcome Co.), Quazi Agashakey, Michelle Kirchner and Sheenah Mische (The Rockefeller University Protein/DNA Technology Center) for sequence analysis of ABRF-95SEQ.",

year = "1996",

doi = "10.1016/S1080-8914(96)80039-1",

language = "English",

volume = "7",

pages = "347--358",

journal = "Techniques in Protein Chemistry",

issn = "1080-8914",

number = "C",

}

TY - JOUR

T1 - Design and analysis of ABRF-95SEQ, a recombinant protein with sequence heterogeneity

AU - De Jongh, Karen S.

AU - Fernandez, Joseph

AU - Gambee, Jay E.

AU - Grant, Gregory A.

AU - Merrill, Barbara

AU - Stone, Kathryn L.

AU - Rush, John

N1 - Funding Information: This work was partially supported by NSF grant DIR9003100 to John Crabb (W. Alton Jones Cell Science Center) on behalf of the ABRF. The contribution of all respondents that made this study possible by analyzing ABRF-95SEQ is gratefully acknowledged. The assistance of Gary P. Gryan (HHMI/Harvard Medical School Computer Facility) in coordinating the data returns and ensuring the anonymity of the participating laboratories is appreciated. The assistance of the ABRF Business Office for postcard printing and mailings is acknowledged. We thank Dr Hong Chen and Dr Geoffrey Duyk for generously providing the pGEX/ocp II expression plasmid. Several members of the authors' laboratories contributed to this project including Dawn Fitzpatrick and Cara Ruble (HHMI/Harvard Medical School) for construction of ocp variants; Ivar Jensen (HHMI/Harvard Medical School) for protein sequence analysis of ocp variants; Jeffry Bondor (Shriners Hospital for Crippled Children) for sample distribution and Bill Chestnut (Burroughs Wellcome Co.), Quazi Agashakey, Michelle Kirchner and Sheenah Mische (The Rockefeller University Protein/DNA Technology Center) for sequence analysis of ABRF-95SEQ.

PY - 1996

Y1 - 1996

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U2 - 10.1016/S1080-8914(96)80039-1

DO - 10.1016/S1080-8914(96)80039-1

M3 - Article

AN - SCOPUS:77957041365

SN - 1080-8914

VL - 7

SP - 347

EP - 358

JO - Techniques in Protein Chemistry

JF - Techniques in Protein Chemistry

IS - C

ER -

Design and analysis of ABRF-95SEQ, a recombinant protein with sequence heterogeneity

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