In: Techniques in Protein Chemistry, Vol. 7, No. C, 1996, p. 347-358.
Research output: Contribution to journal › Article › peer-review
TY - JOUR
T1 - Design and analysis of ABRF-95SEQ, a recombinant protein with sequence heterogeneity
AU - De Jongh, Karen S.
AU - Fernandez, Joseph
AU - Gambee, Jay E.
AU - Grant, Gregory A.
AU - Merrill, Barbara
AU - Stone, Kathryn L.
AU - Rush, John
N1 - Funding Information: This work was partially supported by NSF grant DIR9003100 to John Crabb (W. Alton Jones Cell Science Center) on behalf of the ABRF. The contribution of all respondents that made this study possible by analyzing ABRF-95SEQ is gratefully acknowledged. The assistance of Gary P. Gryan (HHMI/Harvard Medical School Computer Facility) in coordinating the data returns and ensuring the anonymity of the participating laboratories is appreciated. The assistance of the ABRF Business Office for postcard printing and mailings is acknowledged. We thank Dr Hong Chen and Dr Geoffrey Duyk for generously providing the pGEX/ocp II expression plasmid. Several members of the authors' laboratories contributed to this project including Dawn Fitzpatrick and Cara Ruble (HHMI/Harvard Medical School) for construction of ocp variants; Ivar Jensen (HHMI/Harvard Medical School) for protein sequence analysis of ocp variants; Jeffry Bondor (Shriners Hospital for Crippled Children) for sample distribution and Bill Chestnut (Burroughs Wellcome Co.), Quazi Agashakey, Michelle Kirchner and Sheenah Mische (The Rockefeller University Protein/DNA Technology Center) for sequence analysis of ABRF-95SEQ.
PY - 1996
Y1 - 1996
UR - http://www.scopus.com/inward/record.url?scp=77957041365&partnerID=8YFLogxK
U2 - 10.1016/S1080-8914(96)80039-1
DO - 10.1016/S1080-8914(96)80039-1
M3 - Article
AN - SCOPUS:77957041365
SN - 1080-8914
VL - 7
SP - 347
EP - 358
JO - Techniques in Protein Chemistry
JF - Techniques in Protein Chemistry
IS - C