@article{6ea597e521b0476ea097dd89de5852b1,
title = "Derivation of Airway Basal Stem Cells from Human Pluripotent Stem Cells",
abstract = "Hawkins and colleagues report a directed differentiation protocol enabling the derivation of airway basal cells (“iBCs”) from human iPSCs. iBCs recapitulate hallmark stem cell properties of primary basal cells, including self-renewal and multi-lineage differentiation, thus enabling modeling of airway diseases in vitro and repopulation of tracheal xenografts in vivo.",
keywords = "airway regeneration, basal cells, directed differentiation, induced pluripotent stem cells",
author = "Hawkins, {Finn J.} and Shingo Suzuki and Beermann, {Mary Lou} and Cristina Barill{\`a} and Ruobing Wang and Carlos Villacorta-Martin and Andrew Berical and Jean, {J. C.} and {Le Suer}, Jake and Taylor Matte and Chantelle Simone-Roach and Yang Tang and Schlaeger, {Thorsten M.} and Crane, {Ana M.} and Nadine Matthias and Huang, {Sarah X.L.} and Randell, {Scott H.} and Joshua Wu and Spence, {Jason R.} and Gianni Carraro and Stripp, {Barry R.} and Andras Rab and Sorsher, {Eric J.} and Amjad Horani and Brody, {Steven L.} and Davis, {Brian R.} and Kotton, {Darrell N.}",
note = "Funding Information: We thank Brian R. Tilton of the BUSM Flow Cytometry Core and Yuriy Alekseyev of the Boston University School of Medicine (BUSM) Single Cell Sequencing Core, supported by NIH grant 1UL1TR001430 . We also acknowledge Dr. Zhengmei Mao of UTHealth Microscopy Core Facility for histological sample preparation. For facilities management, we are indebted to Greg Miller (CReM Laboratory Manager) and Marianne James (CReM iPSC Core Manager), supported by grants N01 75N92020C00005 and U01TR001810 . The current work was supported by R01HL139799 and Cystic Fibrosis Foundation (CFF) grant HAWKIN17I0 to F.J.H.; Harry Shwachman Cystic Fibrosis Clinical Investigator Award , NIH CHRC K12HD052896 , and Alfred and Gilda Slifka Fund to R.W.; grant U54 DK110805 to T.M.S.; American Thoracic Society Foundation , Primary Ciliary Dyskinesia Foundation , and Kovler Family Foundation awards to A.H.; CFF grants SORSCH 13XX0 and SORSCH 14XX0 to E.J.S.; R01HL146601 and R01HL128370 to S.L.B.; CFF DAVIS15XX1 and DAVIS17XX0 to B.R.D.; NIH R01 HL139876 to B.R.D. and E.J.S.; R01HL122442 , R01HL095993 , U01HL134745 , and U01HL134766 to D.N.K.; and U01HL148692 to D.N.K. and F.J.H. Funding Information: We thank Brian R. Tilton of the BUSM Flow Cytometry Core and Yuriy Alekseyev of the Boston University School of Medicine (BUSM) Single Cell Sequencing Core, supported by NIH grant 1UL1TR001430. We also acknowledge Dr. Zhengmei Mao of UTHealth Microscopy Core Facility for histological sample preparation. For facilities management, we are indebted to Greg Miller (CReM Laboratory Manager) and Marianne James (CReM iPSC Core Manager), supported by grants N01 75N92020C00005 and U01TR001810. The current work was supported by R01HL139799 and Cystic Fibrosis Foundation (CFF) grant HAWKIN17I0 to F.J.H.; Harry Shwachman Cystic Fibrosis Clinical Investigator Award, NIH CHRC K12HD052896, and Alfred and Gilda Slifka Fund to R.W.; grant U54 DK110805 to T.M.S.; American Thoracic Society Foundation, Primary Ciliary Dyskinesia Foundation, and Kovler Family Foundation awards to A.H.; CFF grants SORSCH 13XX0 and SORSCH 14XX0 to E.J.S.; R01HL146601 and R01HL128370 to S.L.B.; CFF DAVIS15XX1 and DAVIS17XX0 to B.R.D.; NIH R01 HL139876 to B.R.D. and E.J.S.; R01HL122442, R01HL095993, U01HL134745, and U01HL134766 to D.N.K.; and U01HL148692 to D.N.K. and F.J.H. F.J.H. S.S. B.R.D. and D.N.K. conceived the work, designed the experiments, and wrote the manuscript. S.S. A.M.C. and B.R.D. designed the targeting strategy and generated the TP63tdTomato iPSCs. F.J.H. S.S. B.R.D. and D.N.K. developed the BC differentiation protocol. F.J.H. S.S. C.B. A.B. M.L.B. R.W. and J.L.S. performed differentiation experiments. C.V.-M. performed bioinformatic analysis of scRNA-seq. Y.T. and T.M.S. generated CF iPSCs and performed gene correction. R.W. generated ALIs from CF iPSCs. S.S. and N.M. performed in vivo experiments. A.R. and E.J.S. performed Ussing chamber analysis. S.X.L.H. provided critical input. S.H.R. provided primary HBECs and critical input. J.W. J.R.S. G.C. and B.R.S. provided scRNA-seq datasets of fetal and adult airway epithelium. A.H. and S.L.B. performed the experiments characterizing cilia in iPSC-derived MCCs, including TEM and CBF. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2021",
month = jan,
day = "7",
doi = "10.1016/j.stem.2020.09.017",
language = "English",
volume = "28",
pages = "79--95.e8",
journal = "Cell Stem Cell",
issn = "1934-5909",
number = "1",
}