This study investigated the relationship between 59 Fe absorption and the anemia associated with aflatoxicosis in commercial broilers. A level of 0 or 5 micrograms of aflatoxin per gram of diet was fed for a 21 day period beginning at 1, 7, 14, or 21 days of age in each of two trials. Packed cell volume (PCV) and percent retention of an oral dose of 59 Fe were determined. The feeding of aflatoxin resulted in an anemia when fed from 0 to 3 or 1 to 4 weeks of age. At the conclusion of these treatment periods, percent 59 Fe retention was significantly reduced from the controls. Aflatoxin when fed from 2 to 5 weeks of age resulted in a slight but significant depression in PCV (P less than .05) with a slight but nonsignificant depression in percent 59 Fe retention. At the conclusion of the 3 to 6 week treatment period both percent iron retention and PCV were greater than controls but not significantly so. The iron retentions of birds separated into high and low PCV groups within each treatment combination were compared. Although the iron retentions of the low PCV groups were lower than those of the high PCV groups, none of the differences were statistically significant. However, Pearson correlations of PCV with percent 59 Fe retention were - .28 (P less than or equal to .02, n = 69) for control birds and - .19 (P less than or equal to .14, n = 58) for birds fed aflatoxin, suggesting a relationship between PCV and iron absorption.