Dependence of 4′-(Hydroxymethyl)-4,5′,8-trimethylpsoralen Photoaddition on the Conformation of Ribonucleic Acid

John F. Thompson; Jean Pierre Bachellerie; John E. Hearst; John E. Hearst; Kathi Hall

doi:10.1021/bi00535a040

Dependence of 4′-(Hydroxymethyl)-4,5′,8-trimethylpsoralen Photoaddition on the Conformation of Ribonucleic Acid

John F. Thompson, Jean Pierre Bachellerie, John E. Hearst, John E. Hearst, Kathi Hall

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Abstract

The photoaddition of 4′-(hydroxymethyl)-4,5′,8-trimethylpsoralen (HMT) to different conformational states of RNA was studied. Poly(U), poly(A,U) (random copolymer), poly(A-U) (alternating copolymer), poly(A)-poly(U) (double stranded), and poly(U)-poly(A)-poly(U) (triple stranded) were reacted with HMT at different temperatures and salt concentrations. The conformation of the polymers was monitored by UV absorption and circular dichroism. It was found that the rate of HMT photoaddition changed dramatically at structural transitions in the RNA. The alternating copolymer poly(A-U) was found to have the highest rate of addition. Low salt and temperature produced maximal incorporation.

Original language	English
Pages (from-to)	1363-1368
Number of pages	6
Journal	Biochemistry
Volume	21
Issue number	6
DOIs	https://doi.org/10.1021/bi00535a040
State	Published - 1982

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title = "Dependence of 4′-(Hydroxymethyl)-4,5′,8-trimethylpsoralen Photoaddition on the Conformation of Ribonucleic Acid",

abstract = "The photoaddition of 4′-(hydroxymethyl)-4,5′,8-trimethylpsoralen (HMT) to different conformational states of RNA was studied. Poly(U), poly(A,U) (random copolymer), poly(A-U) (alternating copolymer), poly(A)-poly(U) (double stranded), and poly(U)-poly(A)-poly(U) (triple stranded) were reacted with HMT at different temperatures and salt concentrations. The conformation of the polymers was monitored by UV absorption and circular dichroism. It was found that the rate of HMT photoaddition changed dramatically at structural transitions in the RNA. The alternating copolymer poly(A-U) was found to have the highest rate of addition. Low salt and temperature produced maximal incorporation.",

author = "Thompson, {John F.} and Bachellerie, {Jean Pierre} and Hearst, {John E.} and Hearst, {John E.} and Kathi Hall",

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T1 - Dependence of 4′-(Hydroxymethyl)-4,5′,8-trimethylpsoralen Photoaddition on the Conformation of Ribonucleic Acid

AU - Thompson, John F.

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AU - Hearst, John E.

AU - Hall, Kathi

PY - 1982

Y1 - 1982

N2 - The photoaddition of 4′-(hydroxymethyl)-4,5′,8-trimethylpsoralen (HMT) to different conformational states of RNA was studied. Poly(U), poly(A,U) (random copolymer), poly(A-U) (alternating copolymer), poly(A)-poly(U) (double stranded), and poly(U)-poly(A)-poly(U) (triple stranded) were reacted with HMT at different temperatures and salt concentrations. The conformation of the polymers was monitored by UV absorption and circular dichroism. It was found that the rate of HMT photoaddition changed dramatically at structural transitions in the RNA. The alternating copolymer poly(A-U) was found to have the highest rate of addition. Low salt and temperature produced maximal incorporation.

AB - The photoaddition of 4′-(hydroxymethyl)-4,5′,8-trimethylpsoralen (HMT) to different conformational states of RNA was studied. Poly(U), poly(A,U) (random copolymer), poly(A-U) (alternating copolymer), poly(A)-poly(U) (double stranded), and poly(U)-poly(A)-poly(U) (triple stranded) were reacted with HMT at different temperatures and salt concentrations. The conformation of the polymers was monitored by UV absorption and circular dichroism. It was found that the rate of HMT photoaddition changed dramatically at structural transitions in the RNA. The alternating copolymer poly(A-U) was found to have the highest rate of addition. Low salt and temperature produced maximal incorporation.

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JO - Biochemistry

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ER -

Dependence of 4′-(Hydroxymethyl)-4,5′,8-trimethylpsoralen Photoaddition on the Conformation of Ribonucleic Acid

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