Demonstration of an endogenous, competitive inhibitor(s) of [3H] diazepam binding in bovine brain

P. J. Marangos, S. M. Paul, P. Greenlaw, F. K. Goodwin, P. Skolnick

Research output: Contribution to journalArticle

52 Scopus citations

Abstract

An endogenous inhibitor(s) of [3H] diazepam binding to synaptosomes has been demonstrated in bovine brain. The inhibitory activity of crude extracts is heat stable, dialyzable, and not affected by ether extraction. Three distinct peaks of inhibitory activity were resolved using Sephadex G-25 chromatography. The lowest molecular weight peak (<700 daltons) had the highest specific inhibitory activity and its inhibition of [3H] diazepam binding was competitive. A similar low molecular weight fraction was not observed in either muscle or liver suggesting that it may be unique to brain. Thin layer chromatography of the Sephadex G-25 fractions revealed a discrete band of inhibitory activity in the two low molecular weight peaks.

Original languageEnglish
Pages (from-to)1893-1900
Number of pages8
JournalLife Sciences
Volume22
Issue number21
DOIs
StatePublished - Jun 5 1978
Externally publishedYes

Fingerprint Dive into the research topics of 'Demonstration of an endogenous, competitive inhibitor(s) of [<sup>3</sup>H] diazepam binding in bovine brain'. Together they form a unique fingerprint.

  • Cite this