Demonstration of an endogenous, competitive inhibitor(s) of [3H] diazepam binding in bovine brain

P. J. Marangos; S. M. Paul; P. Greenlaw; F. K. Goodwin; P. Skolnick

doi:10.1016/0024-3205(78)90476-9

Demonstration of an endogenous, competitive inhibitor(s) of [³H] diazepam binding in bovine brain

P. J. Marangos, S. M. Paul, P. Greenlaw, F. K. Goodwin, P. Skolnick

Washington University School of Medicine

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

An endogenous inhibitor(s) of [³H] diazepam binding to synaptosomes has been demonstrated in bovine brain. The inhibitory activity of crude extracts is heat stable, dialyzable, and not affected by ether extraction. Three distinct peaks of inhibitory activity were resolved using Sephadex G-25 chromatography. The lowest molecular weight peak (<700 daltons) had the highest specific inhibitory activity and its inhibition of [³H] diazepam binding was competitive. A similar low molecular weight fraction was not observed in either muscle or liver suggesting that it may be unique to brain. Thin layer chromatography of the Sephadex G-25 fractions revealed a discrete band of inhibitory activity in the two low molecular weight peaks.

Original language	English
Pages (from-to)	1893-1900
Number of pages	8
Journal	Life Sciences
Volume	22
Issue number	21
DOIs	https://doi.org/10.1016/0024-3205(78)90476-9
State	Published - Jun 5 1978

Access to Document

10.1016/0024-3205(78)90476-9

Cite this

@article{d5c999536a514e57a2927729440ef28c,

title = "Demonstration of an endogenous, competitive inhibitor(s) of [3H] diazepam binding in bovine brain",

abstract = "An endogenous inhibitor(s) of [3H] diazepam binding to synaptosomes has been demonstrated in bovine brain. The inhibitory activity of crude extracts is heat stable, dialyzable, and not affected by ether extraction. Three distinct peaks of inhibitory activity were resolved using Sephadex G-25 chromatography. The lowest molecular weight peak (<700 daltons) had the highest specific inhibitory activity and its inhibition of [3H] diazepam binding was competitive. A similar low molecular weight fraction was not observed in either muscle or liver suggesting that it may be unique to brain. Thin layer chromatography of the Sephadex G-25 fractions revealed a discrete band of inhibitory activity in the two low molecular weight peaks.",

author = "Marangos, {P. J.} and Paul, {S. M.} and P. Greenlaw and Goodwin, {F. K.} and P. Skolnick",

year = "1978",

month = jun,

day = "5",

doi = "10.1016/0024-3205(78)90476-9",

language = "English",

volume = "22",

pages = "1893--1900",

journal = "Life Sciences",

issn = "0024-3205",

number = "21",

}

TY - JOUR

T1 - Demonstration of an endogenous, competitive inhibitor(s) of [3H] diazepam binding in bovine brain

AU - Marangos, P. J.

AU - Paul, S. M.

AU - Greenlaw, P.

AU - Goodwin, F. K.

AU - Skolnick, P.

PY - 1978/6/5

Y1 - 1978/6/5

N2 - An endogenous inhibitor(s) of [3H] diazepam binding to synaptosomes has been demonstrated in bovine brain. The inhibitory activity of crude extracts is heat stable, dialyzable, and not affected by ether extraction. Three distinct peaks of inhibitory activity were resolved using Sephadex G-25 chromatography. The lowest molecular weight peak (<700 daltons) had the highest specific inhibitory activity and its inhibition of [3H] diazepam binding was competitive. A similar low molecular weight fraction was not observed in either muscle or liver suggesting that it may be unique to brain. Thin layer chromatography of the Sephadex G-25 fractions revealed a discrete band of inhibitory activity in the two low molecular weight peaks.

AB - An endogenous inhibitor(s) of [3H] diazepam binding to synaptosomes has been demonstrated in bovine brain. The inhibitory activity of crude extracts is heat stable, dialyzable, and not affected by ether extraction. Three distinct peaks of inhibitory activity were resolved using Sephadex G-25 chromatography. The lowest molecular weight peak (<700 daltons) had the highest specific inhibitory activity and its inhibition of [3H] diazepam binding was competitive. A similar low molecular weight fraction was not observed in either muscle or liver suggesting that it may be unique to brain. Thin layer chromatography of the Sephadex G-25 fractions revealed a discrete band of inhibitory activity in the two low molecular weight peaks.

UR - http://www.scopus.com/inward/record.url?scp=0018143586&partnerID=8YFLogxK

U2 - 10.1016/0024-3205(78)90476-9

DO - 10.1016/0024-3205(78)90476-9

M3 - Article

C2 - 209277

AN - SCOPUS:0018143586

SN - 0024-3205

VL - 22

SP - 1893

EP - 1900

JO - Life Sciences

JF - Life Sciences

IS - 21

ER -

Demonstration of an endogenous, competitive inhibitor(s) of [³H] diazepam binding in bovine brain

Abstract

Access to Document

Other files and links

Fingerprint

Cite this