Deleting the TGF-β receptor attenuates acute proximal tubule injury

Leslie Gewin, Sangeetha Vadivelu, Surekha Neelisetty, Manakan B. Srichai, Paisit Paueksakon, Ambra Pozzi, Raymond C. Harris, Roy Zent

Research output: Contribution to journalArticlepeer-review

55 Scopus citations

Abstract

TGF-β is a profibrotic growth factor in CKD, but its role in modulating the kidney's response to AKI is not well understood. The proximal tubule epithelial cell, which is themain cellular target of AKI, expresses high levels of both TGF-β and its receptors. To determine how TGF-β signaling in this tubular segment affects the response to AKI, we selectively deleted the TGF-β type II receptor in the proximal tubules of mice. This deletion attenuated renal impairment and reduced tubular apoptosis in mercuric chloride-induced injury. In vitro, deficiency of the TGF-β type II receptor protected proximal tubule epithelial cells from hydrogen peroxide-induced apoptosis, which was mediated in part by Smad-dependent signaling. Taken together, these results suggest that TGF-β signaling in the proximal tubule has a detrimental effect on the response to AKI as a result of its proapoptotic effects.

Original languageEnglish
Pages (from-to)2001-2011
Number of pages11
JournalJournal of the American Society of Nephrology
Volume23
Issue number12
DOIs
StatePublished - 2012

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