De novo generation of neuronal cells from the adult mouse brain

L. J. Richards, T. J. Kilpatrick, P. F. Bartlett

Research output: Contribution to journalArticlepeer-review

449 Scopus citations

Abstract

Cells of neuronal morphology, expressing the 150- and 200-kDa neurofilament proteins, were generated in vitro from populations of neural cells dissociated from adult (>60-day-old) mouse brain. Most of these neurons arose from dividing precursors, as demonstrated by the incorporation of [3H]thymidine during the culture period and autoradiography. Neuronal production was optimal under the conditions in which precursors were initially stimulated with basic fibroblast growth factor and then exposed to medium conditioned by an astrocytic cell line, Ast-1, in serum-free medium. Few, if any, neurons arose in control cultures or in cultures kept in serum and fibroblast growth factor. These results suggest that neuronal precursors exist in the adult mammalian brain, but they require discrete epigenetic signals for their proliferation and differentiation.

Original languageEnglish
Pages (from-to)8591-8595
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number18
DOIs
StatePublished - 1992

Keywords

  • Differentiation
  • Fibroblast growth factor
  • Neurofilament
  • Neuronal precursors

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