TY - JOUR
T1 - De novo generation of neuronal cells from the adult mouse brain
AU - Richards, L. J.
AU - Kilpatrick, T. J.
AU - Bartlett, P. F.
PY - 1992
Y1 - 1992
N2 - Cells of neuronal morphology, expressing the 150- and 200-kDa neurofilament proteins, were generated in vitro from populations of neural cells dissociated from adult (>60-day-old) mouse brain. Most of these neurons arose from dividing precursors, as demonstrated by the incorporation of [3H]thymidine during the culture period and autoradiography. Neuronal production was optimal under the conditions in which precursors were initially stimulated with basic fibroblast growth factor and then exposed to medium conditioned by an astrocytic cell line, Ast-1, in serum-free medium. Few, if any, neurons arose in control cultures or in cultures kept in serum and fibroblast growth factor. These results suggest that neuronal precursors exist in the adult mammalian brain, but they require discrete epigenetic signals for their proliferation and differentiation.
AB - Cells of neuronal morphology, expressing the 150- and 200-kDa neurofilament proteins, were generated in vitro from populations of neural cells dissociated from adult (>60-day-old) mouse brain. Most of these neurons arose from dividing precursors, as demonstrated by the incorporation of [3H]thymidine during the culture period and autoradiography. Neuronal production was optimal under the conditions in which precursors were initially stimulated with basic fibroblast growth factor and then exposed to medium conditioned by an astrocytic cell line, Ast-1, in serum-free medium. Few, if any, neurons arose in control cultures or in cultures kept in serum and fibroblast growth factor. These results suggest that neuronal precursors exist in the adult mammalian brain, but they require discrete epigenetic signals for their proliferation and differentiation.
KW - Differentiation
KW - Fibroblast growth factor
KW - Neurofilament
KW - Neuronal precursors
UR - http://www.scopus.com/inward/record.url?scp=0026688533&partnerID=8YFLogxK
U2 - 10.1073/pnas.89.18.8591
DO - 10.1073/pnas.89.18.8591
M3 - Article
C2 - 1528866
AN - SCOPUS:0026688533
SN - 0027-8424
VL - 89
SP - 8591
EP - 8595
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 18
ER -