De novo assembly and analysis of RNA-seq data

Gordon Robertson, Jacqueline Schein, Readman Chiu, Richard Corbett, Matthew Field, Shaun D. Jackman, Karen Mungall, Sam Lee, Hisanaga Mark Okada, Jenny Q. Qian, Malachi Griffith, Anthony Raymond, Nina Thiessen, Timothee Cezard, Yaron S. Butterfield, Richard Newsome, Simon K. Chan, Rong She, Richard Varhol, Baljit KamohAnna Liisa Prabhu, Angela Tam, Yongjun Zhao, Richard A. Moore, Martin Hirst, Marco A. Marra, Steven J.M. Jones, Pamela A. Hoodless, Inanc Birol

Research output: Contribution to journalArticle

556 Scopus citations

Abstract

We describe Trans-ABySS, a de novo short-read transcriptome assembly and analysis pipeline that addresses variation in local read densities by assembling read substrings with varying stringencies and then merging the resulting contigs before analysis. Analyzing 7.4 gigabases of 50-base-pair paired-end Illumina reads from an adult mouse liver poly(A) RNA library, we identified known, new and alternative structures in expressed transcripts, and achieved high sensitivity and specificity relative to reference-based assembly methods.

Original languageEnglish
Pages (from-to)909-912
Number of pages4
JournalNature Methods
Volume7
Issue number11
DOIs
StatePublished - Nov 1 2010
Externally publishedYes

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    Robertson, G., Schein, J., Chiu, R., Corbett, R., Field, M., Jackman, S. D., Mungall, K., Lee, S., Okada, H. M., Qian, J. Q., Griffith, M., Raymond, A., Thiessen, N., Cezard, T., Butterfield, Y. S., Newsome, R., Chan, S. K., She, R., Varhol, R., ... Birol, I. (2010). De novo assembly and analysis of RNA-seq data. Nature Methods, 7(11), 909-912. https://doi.org/10.1038/nmeth.1517