Changes in chondrocyte shape and volume are believed to play a role in the mechanical signal transduction pathway of articular cartilage. To examine how chondrocyte deformation may be transduced to an intracellular signal, confocal microscopy was used to perform three-dimensional morphometric analyses of chondrocyte nuclei during applied deformation of the cartilage matrix. Uniaxial compression of the matrix resulted in a significant decrease in nuclear height in the direction of compression, concomitant with decreases in overall chondrocyte height and volume. Disruption of the actin cytoskeleton resulted in a decrease in nuclear size and prevented nuclear deformation during matrix loading. These findings suggest that the chondrocyte nucleus exists in a state of tensile prestress, and that the actin cytoskeleton plays an important role in regulating nuclear deformation.
|Number of pages||2|
|State||Published - Dec 1 1994|
|Event||Proceedings of the 1994 International Mechanical Engineering Congress and Exposition - Chicago, IL, USA|
Duration: Nov 6 1994 → Nov 11 1994
|Conference||Proceedings of the 1994 International Mechanical Engineering Congress and Exposition|
|City||Chicago, IL, USA|
|Period||11/6/94 → 11/11/94|