TY - JOUR
T1 - Cytolytic T lymphocytes from human renal allograft biopsies are tissue specific
AU - Poindexter, Nancy J.
AU - Steward, Nancy S.
AU - Shenoy, Surendra
AU - Jendrisak, Martin D.
AU - Flye, M. Wayne
AU - Howard, Todd K.
AU - Mohanakumar, T.
N1 - Funding Information:
This work was supportedb y grants from the National Insti-tuteso f Health (DK32253) and the Mid-American Transplant Associationt o T.M., and the National Kidney Foundation to N.J.P. The authorst hank Ms. Billie Glasscockf or her excellent secretariaal ssistanceW. e are grateful to Mr. Brian Duffy for his assistancew ith cytofluorometrya nd immunofluorescence analysis.
PY - 1995/9
Y1 - 1995/9
N2 - The cytolytic activity of T lymphocytes infiltrating renal allografts from recipients undergoing episodes of acute cellular rejection was studied. These T-cell populations, composed of both CD4 + and CD8 + cells, demonstrated significant cytolytic activity against both donor-derived KCLs and B-LCLs. In five of 21 biopsy-derived lines greater cytolytic activity was measured against donor KCLs compared to donor B-LCLs, suggesting the presence of kidney antigen-specific, MHC-restricted clones. Clones developed by stimulation with donor B-LCLs lysed both donor B-LCLs and KCLs while clones developed on donor KCLs as stimulator cells showed tissue specificity. Three of 26 clones recognized tissue-specific antigens in the context of donor MHC class I antigens lysing donor KCLs but not B-LCLs. These data demonstrate that a subpopulation of T cells recognizing kidney-specific antigens are present in biopsies of renal allograft recipients undergoing acute cellular rejection. This subpopulation of tissue-specific cytotoxic T lymphocytes may prove to contribute significantly to the pathology of allograft rejection.
AB - The cytolytic activity of T lymphocytes infiltrating renal allografts from recipients undergoing episodes of acute cellular rejection was studied. These T-cell populations, composed of both CD4 + and CD8 + cells, demonstrated significant cytolytic activity against both donor-derived KCLs and B-LCLs. In five of 21 biopsy-derived lines greater cytolytic activity was measured against donor KCLs compared to donor B-LCLs, suggesting the presence of kidney antigen-specific, MHC-restricted clones. Clones developed by stimulation with donor B-LCLs lysed both donor B-LCLs and KCLs while clones developed on donor KCLs as stimulator cells showed tissue specificity. Three of 26 clones recognized tissue-specific antigens in the context of donor MHC class I antigens lysing donor KCLs but not B-LCLs. These data demonstrate that a subpopulation of T cells recognizing kidney-specific antigens are present in biopsies of renal allograft recipients undergoing acute cellular rejection. This subpopulation of tissue-specific cytotoxic T lymphocytes may prove to contribute significantly to the pathology of allograft rejection.
UR - http://www.scopus.com/inward/record.url?scp=0029122169&partnerID=8YFLogxK
U2 - 10.1016/0198-8859(95)00058-C
DO - 10.1016/0198-8859(95)00058-C
M3 - Article
C2 - 8522454
AN - SCOPUS:0029122169
SN - 0198-8859
VL - 44
SP - 43
EP - 49
JO - Human Immunology
JF - Human Immunology
IS - 1
ER -