Cytolytic T lymphocytes from human renal allograft biopsies are tissue specific

Nancy J. Poindexter, Nancy S. Steward, Surendra Shenoy, Martin D. Jendrisak, M. Wayne Flye, Todd K. Howard, T. Mohanakumar

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28 Scopus citations

Abstract

The cytolytic activity of T lymphocytes infiltrating renal allografts from recipients undergoing episodes of acute cellular rejection was studied. These T-cell populations, composed of both CD4 + and CD8 + cells, demonstrated significant cytolytic activity against both donor-derived KCLs and B-LCLs. In five of 21 biopsy-derived lines greater cytolytic activity was measured against donor KCLs compared to donor B-LCLs, suggesting the presence of kidney antigen-specific, MHC-restricted clones. Clones developed by stimulation with donor B-LCLs lysed both donor B-LCLs and KCLs while clones developed on donor KCLs as stimulator cells showed tissue specificity. Three of 26 clones recognized tissue-specific antigens in the context of donor MHC class I antigens lysing donor KCLs but not B-LCLs. These data demonstrate that a subpopulation of T cells recognizing kidney-specific antigens are present in biopsies of renal allograft recipients undergoing acute cellular rejection. This subpopulation of tissue-specific cytotoxic T lymphocytes may prove to contribute significantly to the pathology of allograft rejection.

Original languageEnglish
Pages (from-to)43-49
Number of pages7
JournalHuman Immunology
Volume44
Issue number1
DOIs
StatePublished - Sep 1995

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    Poindexter, N. J., Steward, N. S., Shenoy, S., Jendrisak, M. D., Flye, M. W., Howard, T. K., & Mohanakumar, T. (1995). Cytolytic T lymphocytes from human renal allograft biopsies are tissue specific. Human Immunology, 44(1), 43-49. https://doi.org/10.1016/0198-8859(95)00058-C