Cytolysin-mediated translocation (CMT): A functional equivalent of type III secretion in Gram-positive bacteria

John C. Madden, Natividad Ruiz, Michael Caparon

Research output: Contribution to journalArticlepeer-review

224 Scopus citations

Abstract

Type III secretion for injection of effector proteins into host cells has not been described for Gram-positive bacteria despite their importance in disease. Here, we describe an injection pathway for the Gram-positive pathogen Streptococcus pyogenes that utilizes streptolysin O (SLO), a cholesterol-dependent cytolysin. The data support a model in which an effector is translocated through the SLO pore by a polarized process. The effector, SPN (S. pyogenes NAD-glycohydrolase), is capable of producing the potent second messenger cyclic ADP-ribose, and SLO and SPN act synergistically to trigger cytotoxicity. These data provide a novel paradigm for the function of the cholesterol-dependent cytolysin family and its wide distribution suggests that cytolysin-mediated translocation (CMT) may be the equivalent of type III secretion for Gram-positive pathogens.

Original languageEnglish
Pages (from-to)143-152
Number of pages10
JournalCell
Volume104
Issue number1
DOIs
StatePublished - Jan 12 2001

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