TY - JOUR
T1 - Cytolysin-mediated translocation (CMT)
T2 - A functional equivalent of type III secretion in Gram-positive bacteria
AU - Madden, John C.
AU - Ruiz, Natividad
AU - Caparon, Michael
N1 - Funding Information:
These studies were supported by Public Health Service Grants AR45254 and AI38273 from the National Institutes of Health. We would like to thank Bruce Roe and colleagues at the Streptococcal Genome Sequencing Project for making their sequencing data available prior to completion.
PY - 2001/1/12
Y1 - 2001/1/12
N2 - Type III secretion for injection of effector proteins into host cells has not been described for Gram-positive bacteria despite their importance in disease. Here, we describe an injection pathway for the Gram-positive pathogen Streptococcus pyogenes that utilizes streptolysin O (SLO), a cholesterol-dependent cytolysin. The data support a model in which an effector is translocated through the SLO pore by a polarized process. The effector, SPN (S. pyogenes NAD-glycohydrolase), is capable of producing the potent second messenger cyclic ADP-ribose, and SLO and SPN act synergistically to trigger cytotoxicity. These data provide a novel paradigm for the function of the cholesterol-dependent cytolysin family and its wide distribution suggests that cytolysin-mediated translocation (CMT) may be the equivalent of type III secretion for Gram-positive pathogens.
AB - Type III secretion for injection of effector proteins into host cells has not been described for Gram-positive bacteria despite their importance in disease. Here, we describe an injection pathway for the Gram-positive pathogen Streptococcus pyogenes that utilizes streptolysin O (SLO), a cholesterol-dependent cytolysin. The data support a model in which an effector is translocated through the SLO pore by a polarized process. The effector, SPN (S. pyogenes NAD-glycohydrolase), is capable of producing the potent second messenger cyclic ADP-ribose, and SLO and SPN act synergistically to trigger cytotoxicity. These data provide a novel paradigm for the function of the cholesterol-dependent cytolysin family and its wide distribution suggests that cytolysin-mediated translocation (CMT) may be the equivalent of type III secretion for Gram-positive pathogens.
UR - http://www.scopus.com/inward/record.url?scp=0035846909&partnerID=8YFLogxK
U2 - 10.1016/S0092-8674(01)00198-2
DO - 10.1016/S0092-8674(01)00198-2
M3 - Article
C2 - 11163247
AN - SCOPUS:0035846909
SN - 0092-8674
VL - 104
SP - 143
EP - 152
JO - Cell
JF - Cell
IS - 1
ER -