Cyp27c1 red-shifts the spectral sensitivity of photoreceptors by converting Vitamin A1 into A2

Jennifer M. Enright; Matthew B. Toomey; Shin Ya Sato; Shelby E. Temple; James R. Allen; Rina Fujiwara; Valerie M. Kramlinger; Leslie D. Nagy; Kevin M. Johnson; Yi Xiao; Martin J. How; Stephen L. Johnson; Nicholas W. Roberts; Vladimir J. Kefalov; F. Peter Guengerich; Joseph C. Corbo

doi:10.1016/j.cub.2015.10.018

Cyp27c1 red-shifts the spectral sensitivity of photoreceptors by converting Vitamin A₁ into A₂

Jennifer M. Enright, Matthew B. Toomey, Shin Ya Sato, Shelby E. Temple, James R. Allen, Rina Fujiwara, Valerie M. Kramlinger, Leslie D. Nagy, Kevin M. Johnson, Yi Xiao, Martin J. How, Stephen L. Johnson, Nicholas W. Roberts, Vladimir J. Kefalov, F. Peter Guengerich, Joseph C. Corbo

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122 Scopus citations

Abstract

Some vertebrate species have evolved means of extending their visual sensitivity beyond the range of human vision. One mechanism of enhancing sensitivity to long-wavelength light is to replace the 11-cis retinal chromophore in photopigments with 11-cis 3,4-didehydroretinal. Despite over a century of research on this topic, the enzymatic basis of this perceptual switch remains unknown. Here, we show that a cytochrome P450 family member, Cyp27c1, mediates this switch by converting vitamin A₁ (the precursor of 11-cis retinal) into vitamin A₂ (the precursor of 11-cis 3,4-didehydroretinal). Knockout of cyp27c1 in zebrafish abrogates production of vitamin A₂, eliminating the animal's ability to red-shift its photoreceptor spectral sensitivity and reducing its ability to see and respond to near-infrared light. Thus, the expression of a single enzyme mediates dynamic spectral tuning of the entire visual system by controlling the balance of vitamin A₁ and A₂ in the eye.

Original language	English
Pages (from-to)	3048-3057
Number of pages	10
Journal	Current Biology
Volume	25
Issue number	23
DOIs	https://doi.org/10.1016/j.cub.2015.10.018
State	Published - Dec 7 2015

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Enright, J. M., Toomey, M. B., Sato, S. Y., Temple, S. E., Allen, J. R., Fujiwara, R., Kramlinger, V. M., Nagy, L. D., Johnson, K. M., Xiao, Y., How, M. J., Johnson, S. L., Roberts, N. W., Kefalov, V. J., Peter Guengerich, F., & Corbo, J. C. (2015). Cyp27c1 red-shifts the spectral sensitivity of photoreceptors by converting Vitamin A₁ into A₂. Current Biology, 25(23), 3048-3057. https://doi.org/10.1016/j.cub.2015.10.018

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title = "Cyp27c1 red-shifts the spectral sensitivity of photoreceptors by converting Vitamin A1 into A2",

abstract = "Some vertebrate species have evolved means of extending their visual sensitivity beyond the range of human vision. One mechanism of enhancing sensitivity to long-wavelength light is to replace the 11-cis retinal chromophore in photopigments with 11-cis 3,4-didehydroretinal. Despite over a century of research on this topic, the enzymatic basis of this perceptual switch remains unknown. Here, we show that a cytochrome P450 family member, Cyp27c1, mediates this switch by converting vitamin A1 (the precursor of 11-cis retinal) into vitamin A2 (the precursor of 11-cis 3,4-didehydroretinal). Knockout of cyp27c1 in zebrafish abrogates production of vitamin A2, eliminating the animal's ability to red-shift its photoreceptor spectral sensitivity and reducing its ability to see and respond to near-infrared light. Thus, the expression of a single enzyme mediates dynamic spectral tuning of the entire visual system by controlling the balance of vitamin A1 and A2 in the eye.",

author = "Enright, {Jennifer M.} and Toomey, {Matthew B.} and Sato, {Shin Ya} and Temple, {Shelby E.} and Allen, {James R.} and Rina Fujiwara and Kramlinger, {Valerie M.} and Nagy, {Leslie D.} and Johnson, {Kevin M.} and Yi Xiao and How, {Martin J.} and Johnson, {Stephen L.} and Roberts, {Nicholas W.} and Kefalov, {Vladimir J.} and {Peter Guengerich}, F. and Corbo, {Joseph C.}",

year = "2015",

month = dec,

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doi = "10.1016/j.cub.2015.10.018",

language = "English",

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Enright, JM, Toomey, MB, Sato, SY, Temple, SE, Allen, JR, Fujiwara, R, Kramlinger, VM, Nagy, LD, Johnson, KM, Xiao, Y, How, MJ, Johnson, SL, Roberts, NW, Kefalov, VJ, Peter Guengerich, F & Corbo, JC 2015, 'Cyp27c1 red-shifts the spectral sensitivity of photoreceptors by converting Vitamin A₁ into A₂', Current Biology, vol. 25, no. 23, pp. 3048-3057. https://doi.org/10.1016/j.cub.2015.10.018

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T1 - Cyp27c1 red-shifts the spectral sensitivity of photoreceptors by converting Vitamin A1 into A2

AU - Enright, Jennifer M.

AU - Toomey, Matthew B.

AU - Sato, Shin Ya

AU - Temple, Shelby E.

AU - Allen, James R.

AU - Fujiwara, Rina

AU - Kramlinger, Valerie M.

AU - Nagy, Leslie D.

AU - Johnson, Kevin M.

AU - Xiao, Yi

AU - How, Martin J.

AU - Johnson, Stephen L.

AU - Roberts, Nicholas W.

AU - Kefalov, Vladimir J.

AU - Peter Guengerich, F.

AU - Corbo, Joseph C.

PY - 2015/12/7

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AB - Some vertebrate species have evolved means of extending their visual sensitivity beyond the range of human vision. One mechanism of enhancing sensitivity to long-wavelength light is to replace the 11-cis retinal chromophore in photopigments with 11-cis 3,4-didehydroretinal. Despite over a century of research on this topic, the enzymatic basis of this perceptual switch remains unknown. Here, we show that a cytochrome P450 family member, Cyp27c1, mediates this switch by converting vitamin A1 (the precursor of 11-cis retinal) into vitamin A2 (the precursor of 11-cis 3,4-didehydroretinal). Knockout of cyp27c1 in zebrafish abrogates production of vitamin A2, eliminating the animal's ability to red-shift its photoreceptor spectral sensitivity and reducing its ability to see and respond to near-infrared light. Thus, the expression of a single enzyme mediates dynamic spectral tuning of the entire visual system by controlling the balance of vitamin A1 and A2 in the eye.

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SP - 3048

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JF - Current Biology

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ER -

Cyp27c1 red-shifts the spectral sensitivity of photoreceptors by converting Vitamin A₁ into A₂

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