Cyclosporine a inhibits lymphocyte migration into ovine peripheral nerve allografts

Gregory M.T. Hare, Susan E. Mackinnon, Rajiv Midha, Pui Yeun Wong, Bihn Au, Catherine Munro, William Andrade, Daniel A. Hunter, John B. Hay

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Lymphocyte migration into nerve allografts was measured to estimate the cyclosporine A (CsA) dose required to suppress rejection. Twelve outbred sheep received daily subcutaneous CsA at 0, 5, 10, or 15 mg/kg/day for 2 weeks prior to implantation of multiple heterotopic subcutaneous nerve grafts. Lymphocyte migration was determined after 7 days by an intravenous pulse of autologous 111indium-labeled lymphocytes and subsequent quantitation of gamma radioactivity in nerve tissue (CPM/g, mean ± SEM). Measurement by radioimmunoassay revealed a dose-dependent increase in blood cyclosporine levels. Lymphocyte migration into autografts (404 ± 44) was significantly less than migration into allografts (16,554 ± 2,049), in control animals (P < 0.01). A dose-dependent inhibition of lymphocyte migration into nerve allografts was observed with counts of 7,662 ± 1,692, 4,083 ± 1,112, and 1,561 ± 232 in sheep receiving 5, 10, or 15 mg/kg/day of CsA, respectively. Daily CsA administration produced effective blood levels and immunosuppression sufficient to inhibit lymphocyte migration into nerve allografts.

Original languageEnglish
Pages (from-to)697-705
Number of pages9
JournalMicrosurgery
Volume17
Issue number12
DOIs
StatePublished - 1996
Externally publishedYes

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