TY - JOUR
T1 - Cyclopentenone isoprostanes inhibit the inflammatory response in macrophages
AU - Musiek, Erik S.
AU - Gao, Ling
AU - Milne, Ginger L.
AU - Han, Wei
AU - Everhart, M. Brett
AU - Wang, Dingzhi
AU - Backlund, Michael G.
AU - DuBois, Raymond N.
AU - Zanoni, Giuseppe
AU - Vidari, Giovanni
AU - Blackwell, Timothy S.
AU - Morrow, Jason D.
PY - 2005/10/21
Y1 - 2005/10/21
N2 - Although both inflammation and oxidative stress contribute to the pathogenesis of many disease states, the interaction between the two is poorly understood. Cyclopentenone isoprostanes (IsoPs), highly reactive structural isomers of the bioactive cyclopentenone prostaglandins PGA2 and PGJ2, are formed non-enzymatically as products of oxidative stress in vivo. We have, for the first time, examined the effects of synthetic 15-A 2- and 15-J2-IsoPs, two groups of endogenous cyclopentenone IsoPs, on the inflammatory response in RAW264.7 and primary murine macrophages. Cyclopentenone IsoPs potently inhibited lipopolysaccharide- stimulated IκBα degradation and subsequent NF-κB nuclear translocation and transcriptional activity. Expression of inducible nitric-oxide synthase and cyclooxygenase-2 were also inhibited by cyclopentenone IsoPs as was nitrite and prostaglandin production (IC50 ∼ 360 and 210 nM, respectively). 15-J2-IsoPs potently activated peroxisome proliferator-activated receptor γ (PPARγ) nuclear receptors, whereas 15-A2-IsoP did not, although the anti-inflammatory effects of both molecules were PPARγ-independent. Interestingly 15-A2-IsoPs induced oxidative stress in RAW cells that was blocked by the antioxidant 4-hydroxy-TEMPO (TEMPOL) or the mitochondrial uncoupler carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone. TEMPOL also abrogated the inhibitory effect of 15-A2-IsoPs on lipopolysaccharide-induced NF-κB activation, inducible nitric-oxide synthase expression, and nitrite production, suggesting that 15-A2-IsoPs inhibit the NF-κB pathway at least partially via a redox-dependent mechanism. 15-J2-IsoP, but not 15-A 2-IsoP, also potently induced RAW cell apoptosis again via a PPARγ-independent mechanism. These findings suggest that cyclopentenone IsoPs may serve as negative feedback regulators of inflammation and have important implications for defining the role of oxidative stress in the inflammatory response.
AB - Although both inflammation and oxidative stress contribute to the pathogenesis of many disease states, the interaction between the two is poorly understood. Cyclopentenone isoprostanes (IsoPs), highly reactive structural isomers of the bioactive cyclopentenone prostaglandins PGA2 and PGJ2, are formed non-enzymatically as products of oxidative stress in vivo. We have, for the first time, examined the effects of synthetic 15-A 2- and 15-J2-IsoPs, two groups of endogenous cyclopentenone IsoPs, on the inflammatory response in RAW264.7 and primary murine macrophages. Cyclopentenone IsoPs potently inhibited lipopolysaccharide- stimulated IκBα degradation and subsequent NF-κB nuclear translocation and transcriptional activity. Expression of inducible nitric-oxide synthase and cyclooxygenase-2 were also inhibited by cyclopentenone IsoPs as was nitrite and prostaglandin production (IC50 ∼ 360 and 210 nM, respectively). 15-J2-IsoPs potently activated peroxisome proliferator-activated receptor γ (PPARγ) nuclear receptors, whereas 15-A2-IsoP did not, although the anti-inflammatory effects of both molecules were PPARγ-independent. Interestingly 15-A2-IsoPs induced oxidative stress in RAW cells that was blocked by the antioxidant 4-hydroxy-TEMPO (TEMPOL) or the mitochondrial uncoupler carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone. TEMPOL also abrogated the inhibitory effect of 15-A2-IsoPs on lipopolysaccharide-induced NF-κB activation, inducible nitric-oxide synthase expression, and nitrite production, suggesting that 15-A2-IsoPs inhibit the NF-κB pathway at least partially via a redox-dependent mechanism. 15-J2-IsoP, but not 15-A 2-IsoP, also potently induced RAW cell apoptosis again via a PPARγ-independent mechanism. These findings suggest that cyclopentenone IsoPs may serve as negative feedback regulators of inflammation and have important implications for defining the role of oxidative stress in the inflammatory response.
UR - http://www.scopus.com/inward/record.url?scp=27444436893&partnerID=8YFLogxK
U2 - 10.1074/jbc.M504785200
DO - 10.1074/jbc.M504785200
M3 - Article
C2 - 16100121
AN - SCOPUS:27444436893
SN - 0021-9258
VL - 280
SP - 35562
EP - 35570
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 42
ER -