cAMP and cGMP phosphodiesterase (PDE) activity was assayed in human peripheral blood lymphocytes purified by isopycnic centrifugation as well as in lymphocyte preparations further purified to remove contaminating platelets and monocytes. The 16,000xG supernatant from sonicates of each of these cell preparations contained two hydrolytic activities for cAMP with apparent K(m) of 1.1 to 2.5 μM and 33 to 66 μM, and a single hydrolytic activity for cGMP with an apparent K(m) of 6 to 25 μM. When lymphocytes were disrupted by Dounce homogenization, there was only a single, low K(m) cAMP PDE activity in the homogenate; however, the 16,000xG supernatant demonstrated 2 K(m) similar to that seen in sonicated lymphocytes. Treatment of the Dounce preparations with 0.5% Triton X-100 or 1.0% NP-40 converted these preparations to activities similar to those seen in sonicated preparations. cGMP hydrolytic activity was low or absent in the Dounce preparations and was not altered by centrifugation; however, it was markedly enhanced by detergent extraction. These data indicate that human peripheral blood lymphocytes and monocytes have PDE activities similar to those seen in other tissues.
|Number of pages||8|
|Journal||Journal of Immunology|
|State||Published - Dec 1 1979|