CXCL5 is required for angiogenesis, but not structural adaptation after small bowel resection

Kathryn J. Rowland, Jose Diaz-Miron, Jun Guo, Christopher R. Erwin, Junjie Mei, G. Scott Worthen, Brad W. Warner

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


Purpose Intestinal adaptation is the compensatory response to massive small bowel resection (SBR) and characterized by lengthening of villi and deepening of crypts, resulting in increased mucosal surface area. Previous studies have demonstrated increased villus capillary blood vessel density after SBR, suggesting a role for angiogenesis in the development of resection-induced adaptation. Since we have previously shown enhanced expression of the proangiogenic chemokine CXCL5 after SBR, the purpose of this study was to determine the effect of disrupted CXCL5 expression on intestinal adaptation. Methods CXCL5 knockout (KO) and C57BL/6 wild type (WT) mice were subjected to either a 50% proximal SBR or sham operation. Ileal tissue was harvested on postoperative day 7. To assess for adaptation, villus height and crypt depth were measured. Submucosal capillary density was measured by CD31 immunohistochemistry. Results Both CXCL5-KO and WT mice demonstrated normal structural features of adaptation. Submucosal capillary density increased in the WT but not in the KO mice following SBR. Conclusion CXCL5 is required for increased intestinal angiogenesis during resection-induced adaptation. Since adaptive villus growth occurs despite impaired CXCL5 expression and enhanced angiogenesis, this suggests that the growth of new blood vessels is not needed for resection-induced mucosal surface area expansion following massive SBR.

Original languageEnglish
Pages (from-to)976-980
Number of pages5
JournalJournal of Pediatric Surgery
Issue number6
StatePublished - Jun 2014


  • Adaptation
  • Angiogenesis
  • CXCL5
  • Intestine
  • Small bowel resection


Dive into the research topics of 'CXCL5 is required for angiogenesis, but not structural adaptation after small bowel resection'. Together they form a unique fingerprint.

Cite this