Cutting edge: The histone methyltransferase G9a is required for silencing of helper T lineage-associated genes in proliferating CD8 T cells

Daniel J. Verbaro, Nagisa Sakurai, Byungil Kim, Yoichi Shinkai, Takeshi Egawa

Research output: Contribution to journalReview article

4 Scopus citations

Abstract

Helper versus cytotoxic T lineage decision in the thymus has been studied as a model for silencing of alternative lineage genes. Although the transcription factor RUNX3 is required for the initiation of Cd4 silencing in developing CD8 T cells, it is unknown how silencing of Cd4 and other helper T lineage genes is maintained. We show that the histone methyltransferase G9a is necessary for silencing helper T lineage genes in proliferating mouse CD8 T cells. Despite normal initial Cd4 downregulation, G9a-deficient CD8 T cells derepress Cd4 and other helper lineage genes during repeated division in lymphopenia or in response to tumor Ag. However, G9a was dispensable for continued silencing of those genes in CD8 T cells that respond to infection by Listeria monocytogenes. These results demonstrate that G9a facilitates maintenance of cellular identity of CD8 T cells during cell division, which is further reinforced by inflammatory signals.

Original languageEnglish
Pages (from-to)3891-3896
Number of pages6
JournalJournal of Immunology
Volume200
Issue number12
DOIs
StatePublished - Jun 15 2018

Fingerprint Dive into the research topics of 'Cutting edge: The histone methyltransferase G9a is required for silencing of helper T lineage-associated genes in proliferating CD8 T cells'. Together they form a unique fingerprint.

  • Cite this