Cutting edge: Signal-regulatory protein β1 is a DAP12-associated activating receptor expressed in myeloid cells

Jes Dietrich, Marina Cella, Martina Seiffert, Hans Jörg Bühring, Marco Colonna

Research output: Contribution to journalArticlepeer-review

127 Scopus citations

Abstract

Signal-regulatory proteins (SIRPs) are cell-surface glycoproteins expressed on myeloid and neural cells that have been shown to recruit SH2 domain-containing protein phosphatase 1 (SHP-1) and SHP-2 and to regulate receptor tyrosine kinase-coupled signaling. One SIRP of unknown function, designated SIRPβ1, contains a short cytoplasmic domain that lacks sequence motifs capable of recruiting SHP-1 and SHP-2. Using a SIRP-specific mAb, we show that SIRPβ1 is expressed in monocytes and dendritic cells and associates with the signal transduction molecule DAP12. SIRPβ1/DAP12 complex formation was required for efficient cell-surface expression of SIRPβ1. Stimulation of this complex induced tyrosine phosphorylation, mitogen- activated protein kinase activation, and cellular activation. Thus, SIRPβ1 is a new DAP12-associated receptor involved in the activation of myeloid cells.

Original languageEnglish
Pages (from-to)9-12
Number of pages4
JournalJournal of Immunology
Volume164
Issue number1
DOIs
StatePublished - Jan 1 2000

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