TY - JOUR
T1 - Cutting edge
T2 - Signal-regulatory protein β1 is a DAP12-associated activating receptor expressed in myeloid cells
AU - Dietrich, Jes
AU - Cella, Marina
AU - Seiffert, Martina
AU - Bühring, Hans Jörg
AU - Colonna, Marco
N1 - Funding Information:
This study was supported by grants from the Swedish National Heart and Lung Foundation, the Wiberg Foundation, the Gun and Bertil Stohne Foundation, the Karolinska Institute and the Swedish Medical Association.
PY - 2000/1/1
Y1 - 2000/1/1
N2 - Signal-regulatory proteins (SIRPs) are cell-surface glycoproteins expressed on myeloid and neural cells that have been shown to recruit SH2 domain-containing protein phosphatase 1 (SHP-1) and SHP-2 and to regulate receptor tyrosine kinase-coupled signaling. One SIRP of unknown function, designated SIRPβ1, contains a short cytoplasmic domain that lacks sequence motifs capable of recruiting SHP-1 and SHP-2. Using a SIRP-specific mAb, we show that SIRPβ1 is expressed in monocytes and dendritic cells and associates with the signal transduction molecule DAP12. SIRPβ1/DAP12 complex formation was required for efficient cell-surface expression of SIRPβ1. Stimulation of this complex induced tyrosine phosphorylation, mitogen- activated protein kinase activation, and cellular activation. Thus, SIRPβ1 is a new DAP12-associated receptor involved in the activation of myeloid cells.
AB - Signal-regulatory proteins (SIRPs) are cell-surface glycoproteins expressed on myeloid and neural cells that have been shown to recruit SH2 domain-containing protein phosphatase 1 (SHP-1) and SHP-2 and to regulate receptor tyrosine kinase-coupled signaling. One SIRP of unknown function, designated SIRPβ1, contains a short cytoplasmic domain that lacks sequence motifs capable of recruiting SHP-1 and SHP-2. Using a SIRP-specific mAb, we show that SIRPβ1 is expressed in monocytes and dendritic cells and associates with the signal transduction molecule DAP12. SIRPβ1/DAP12 complex formation was required for efficient cell-surface expression of SIRPβ1. Stimulation of this complex induced tyrosine phosphorylation, mitogen- activated protein kinase activation, and cellular activation. Thus, SIRPβ1 is a new DAP12-associated receptor involved in the activation of myeloid cells.
UR - http://www.scopus.com/inward/record.url?scp=0033981961&partnerID=8YFLogxK
U2 - 10.4049/jimmunol.164.1.9
DO - 10.4049/jimmunol.164.1.9
M3 - Article
C2 - 10604985
AN - SCOPUS:0033981961
SN - 0022-1767
VL - 164
SP - 9
EP - 12
JO - Journal of Immunology
JF - Journal of Immunology
IS - 1
ER -