CUT&Tag2for1: a modified method for simultaneous profiling of the accessible and silenced regulome in single cells

Derek H. Janssens, Dominik J. Otto, Michael P. Meers, Manu Setty, Kami Ahmad, Steven Henikoff

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Cleavage Under Targets and Tagmentation (CUT&Tag) is an antibody-directed transposase tethering strategy for in situ chromatin profiling in small samples and single cells. We describe a modified CUT&Tag protocol using a mixture of an antibody to the initiation form of RNA polymerase II (Pol2 Serine-5 phosphate) and an antibody to repressive Polycomb domains (H3K27me3) followed by computational signal deconvolution to produce high-resolution maps of both the active and repressive regulomes in single cells. The ability to seamlessly map active promoters, enhancers, and repressive regulatory elements using a single workflow provides a complete regulome profiling strategy suitable for high-throughput single-cell platforms.

Original languageEnglish
Article number81
JournalGenome biology
Volume23
Issue number1
DOIs
StatePublished - Dec 2022

Keywords

  • Chromatin profiling
  • Epigenomic signal deconvolution
  • Multi-omics
  • Single-cell genomics

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