Abstract

Mesothelial cells line the serosal cavities and associated organs. In order to metastasize to distant organs, ovarian tumor cells must first attach and then clear the mesothelial cells. Therefore, human primary mesothelial cells (HPMCs) are necessary to effectively study ovarian cancer metastases. Here, we describe methods to obtain HPMCs from human omentum and to culture in vitro.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages147-154
Number of pages8
DOIs
StatePublished - 2022

Publication series

NameMethods in Molecular Biology
Volume2424
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Culture
  • Human omentum
  • Mesothelial cells
  • Ovarian cancer
  • Primary mesothelial cells

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