Cultured chicken embryo lens cells resemble differentiating fiber cells in vivo and contain two kinetic pools of connexin56

Viviana M. Berthoud, Steven Bassnett, Eric C. Beyer

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

The lens is an avascular organ in which gap junctions play a pivotal role for cell physiology and transparency. Here we evaluate a lens culture system as a model for studies of lens gap junction dynamics. In culture, chicken embryo lens cells initially form a monolayer of epithelial cells. Subsequently, the epithelial cells differentiate into lentoids, birefringent multicellular structures composed of fiber-like cells. We examined the cultures for the expression of cellular markers and lens fiber specific proteins using immunofluorescence and immunoblot analysis. We also determined the half-life of connexin56 (Cx56), a fiber-specific gap junction protein. All lens cells in culture expressed actin, endoplasmic reticulum proteins and N-cadherin. Only lentoid cells expressed the lens fiber connexins, Cx45.6 and Cx56. Cx56 localized at appositional membranes and did not co-localize with endoplasmic reticulum proteins or N-cadherin. Two pools of Cx56 were detected in these cultures, one with a half-life of a few hours and the other with a half-life of days. The two pools contained phosphorylated forms of Cx56 of different apparent molecular weights. These results suggest that lens cells in culture can be used as a model for the study of lens biology. They also suggest that phosphorylation of Cx56 might be regulating the stability of the protein.

Original languageEnglish
Pages (from-to)475-484
Number of pages10
JournalExperimental eye research
Volume68
Issue number4
DOIs
StatePublished - Apr 1999

Keywords

  • Connexin
  • Endoplasmic reticulum
  • Gap junction
  • Lens
  • N-cadherin
  • Turnover

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