Culture and differentiation of mouse tracheal epithelial cells

Yingjian You, Steven L. Brody

Research output: Chapter in Book/Report/Conference proceedingChapter

54 Scopus citations

Abstract

Airway epithelial cell biology has been greatly advanced by studies of genetically defined and modified mice; however it is often difficult to isolate, manipulate, and assay epithelial cell-specific responses in vivo. In vitro proliferation and differentiation of mouse airway epithelial cells are made possible by a high-fidelity system for primary culture of mouse tracheal epithelial cells described in this chapter. Using this method, epithelial cells purified from mouse tracheas proliferate in growth factor-enriched medium. Subsequent culture in defined medium and the use of the air-liquid interface condition result in the development of well-differentiated epithelia composed of ciliated and non-ciliated cells with characteristics of native airways. Methods are also provided for manipulation of differentiation and analysis of differentiation and gene expression. These approaches allow the assessment of global responses and those of specific cell subpopulations within the airway epithelium.

Original languageEnglish
Title of host publicationEpithelial Cell Culture Protocols
Subtitle of host publicationSecond Edition
EditorsScott Randell, Leslie Fulcher
Pages123-143
Number of pages21
DOIs
StatePublished - Jan 1 2013

Publication series

NameMethods in Molecular Biology
Volume945
ISSN (Print)1064-3745

Keywords

  • Airway epithelium
  • Cilia
  • Culture
  • Mouse
  • Mucous
  • Trachea

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    You, Y., & Brody, S. L. (2013). Culture and differentiation of mouse tracheal epithelial cells. In S. Randell, & L. Fulcher (Eds.), Epithelial Cell Culture Protocols: Second Edition (pp. 123-143). (Methods in Molecular Biology; Vol. 945). https://doi.org/10.1007/978-1-62703-125-7_9