CtIP Links DNA Double-Strand Break Sensing to Resection

Zhongsheng You, Linda Z. Shi, Quan Zhu, Peng Wu, You Wei Zhang, Andrew Basilio, Nina Tonnu, Inder M. Verma, Michael W. Berns, Tony Hunter

Research output: Contribution to journalArticlepeer-review

166 Scopus citations

Abstract

In response to DNA double-strand breaks (DSBs), cells sense the DNA lesions and then activate the protein kinase ATM. Subsequent DSB resection produces RPA-coated ssDNA that is essential for activation of the DNA damage checkpoint and DNA repair by homologous recombination (HR). However, the biochemical mechanism underlying the transition from DSB sensing to resection remains unclear. Using Xenopus egg extracts and human cells, we show that the tumor suppressor protein CtIP plays a critical role in this transition. We find that CtIP translocates to DSBs, a process dependent on the DSB sensor complex Mre11-Rad50-NBS1, the kinase activity of ATM, and a direct DNA-binding motif in CtIP, and then promotes DSB resection. Thus, CtIP facilitates the transition from DSB sensing to processing: it does so by binding to the DNA at DSBs after DSB sensing and ATM activation and then promoting DNA resection, leading to checkpoint activation and HR.

Original languageEnglish
Pages (from-to)954-969
Number of pages16
JournalMolecular cell
Volume36
Issue number6
DOIs
StatePublished - Dec 24 2009

Keywords

  • DNA
  • PROTEINS

Fingerprint

Dive into the research topics of 'CtIP Links DNA Double-Strand Break Sensing to Resection'. Together they form a unique fingerprint.

Cite this