Crystallographic structure of porcine adenovirus type 4 fiber head and galectin domains

Pablo Guardado-Calvo, Eva M. Muñoz, Antonio L. Llamas-Saiz, Gavin C. Fox, Richard Kahn, David T. Curiel, Joel N. Glasgow, Mark J. Van Raaij

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Adenovirus isolate NADC-1, a strain of porcine adenovirus type 4, has a fiber containing an N-terminal virus attachment region, shaft and head domains, and a C-terminal galectin domain connected to the head by an RGD-containing sequence. The crystal structure of the head domain is similar to previously solved adenovirus fiber head domains, but specific residues for binding the coxsackievirus and adenovirus receptor (CAR), CD46, or sialic acid are not conserved. The structure of the galectin domain reveals an interaction interface between its two carbohydrate recognition domains, locating both sugar binding sites face to face. Sequence evidence suggests other tandem-repeat galectins have the same arrangement. We show that the galectin domain binds carbohydrates containing lactose and N-acetyl-lactosamine units, and we present structures of the galectin domain with lactose, N-acetyl-lactosamine, 3-aminopropyl-lacto-N- neotetraose, and 2-aminoethyl-tri(N-acetyl-lactosamine), confirming the domain as a bona fide galectin domain.

Original languageEnglish
Pages (from-to)10558-10568
Number of pages11
JournalJournal of virology
Volume84
Issue number20
DOIs
StatePublished - Oct 1 2010
Externally publishedYes

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