Crystallization of rat intestinal fatty acid binding protein. Preliminary X-ray data obtained from protein expressed in Escherichia coli.

J. C. Sacchettini, T. A. Meininger, J. B. Lowe, J. I. Gordon, L. J. Banaszak

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Abstract

Rat intestinal fatty acid binding protein has been expressed in Escherichia coli, purified with bound long chain fatty acids and crystals grown from solutions of polyethylene glycol 4000. The crystals are monoclinic, space group P2(1), a = 3638 A, b = 57.2 A, c = 31.9 A, and beta = 113.9 degrees. Each unit cell contains two monomers of this 132-residue, 15.1-kDa polypeptide. The crystals are remarkably resistant to x-ray damage. X-ray diffraction data have been observed to 2.0 A resolution. Platinum chloride was used to generate a potential isomorphous heavy atom derivative.

Original languageEnglish
Pages (from-to)5428-5430
Number of pages3
JournalJournal of Biological Chemistry
Volume262
Issue number11
StatePublished - Apr 15 1987

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