TY - JOUR
T1 - Cryo-EM analysis of Ebola virus nucleocapsid-like assembly
AU - Wang, Yan
AU - Binning, Jennifer M.
AU - Pintilie, Grigore D.
AU - Chiu, Wah
AU - Amarasinghe, Gaya K.
AU - Leung, Daisy W.
AU - Su, Zhaoming
N1 - Publisher Copyright:
© 2021 The Author(s)
PY - 2022/3/18
Y1 - 2022/3/18
N2 - This protocol describes the reconstitution of the filamentous Ebola virus nucleocapsid-like assembly in vitro. This is followed by solving the cryo-EM structure using helical reconstruction, and flexible fitting of the existing model into the 5.8 Å cryo-EM map. The protocol can be applied to other filamentous viral protein assemblies, particularly those with high flexibility and moderate resolution maps, which present technical challenges to model building. For complete details on the use and execution of this profile, please refer to Su et al. (2018).
AB - This protocol describes the reconstitution of the filamentous Ebola virus nucleocapsid-like assembly in vitro. This is followed by solving the cryo-EM structure using helical reconstruction, and flexible fitting of the existing model into the 5.8 Å cryo-EM map. The protocol can be applied to other filamentous viral protein assemblies, particularly those with high flexibility and moderate resolution maps, which present technical challenges to model building. For complete details on the use and execution of this profile, please refer to Su et al. (2018).
KW - Cryo-EM
KW - Microbiology
KW - Protein Biochemistry
KW - Protein expression and purification
KW - Structural Biology
UR - http://www.scopus.com/inward/record.url?scp=85121129831&partnerID=8YFLogxK
U2 - 10.1016/j.xpro.2021.101030
DO - 10.1016/j.xpro.2021.101030
M3 - Article
C2 - 34977676
AN - SCOPUS:85121129831
SN - 2666-1667
VL - 3
JO - STAR Protocols
JF - STAR Protocols
IS - 1
M1 - 101030
ER -