Cryo-EM analysis of Ebola virus nucleocapsid-like assembly

Yan Wang, Jennifer M. Binning, Grigore D. Pintilie, Wah Chiu, Gaya K. Amarasinghe, Daisy W. Leung, Zhaoming Su

Research output: Contribution to journalArticlepeer-review

Abstract

This protocol describes the reconstitution of the filamentous Ebola virus nucleocapsid-like assembly in vitro. This is followed by solving the cryo-EM structure using helical reconstruction, and flexible fitting of the existing model into the 5.8 Å cryo-EM map. The protocol can be applied to other filamentous viral protein assemblies, particularly those with high flexibility and moderate resolution maps, which present technical challenges to model building. For complete details on the use and execution of this profile, please refer to Su et al. (2018).

Original languageEnglish
Article number101030
JournalSTAR Protocols
Volume3
Issue number1
DOIs
StatePublished - Mar 18 2022

Keywords

  • Cryo-EM
  • Microbiology
  • Protein Biochemistry
  • Protein expression and purification
  • Structural Biology

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