CRISPR/Cas9 Guide RNA Design Rules for Predicting Activity

Kasidet Hiranniramol, Yuhao Chen, Xiaowei Wang

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

9 Scopus citations


A critical stage in performing gene editing experiments using the CRISPR/Cas9 system is the design of guide RNA (gRNA). In this chapter, we conduct a review of the current gRNA design rules for maximizing on-target Cas9 activity while minimizing off-target activity. In addition, we present some of the currently available computational tools for gRNA activity prediction and assay design.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages14
StatePublished - 2020

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Cas9
  • Genome editing
  • Machine learning
  • sgRNA


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