@inbook{60f8a29c917d4e12b5434c0d1b24762f,
title = "CRISPR/Cas9 Guide RNA Design Rules for Predicting Activity",
abstract = "A critical stage in performing gene editing experiments using the CRISPR/Cas9 system is the design of guide RNA (gRNA). In this chapter, we conduct a review of the current gRNA design rules for maximizing on-target Cas9 activity while minimizing off-target activity. In addition, we present some of the currently available computational tools for gRNA activity prediction and assay design.",
keywords = "CRISPR, Cas9, Genome editing, Machine learning, sgRNA",
author = "Kasidet Hiranniramol and Yuhao Chen and Xiaowei Wang",
note = "Publisher Copyright: {\textcopyright} 2020, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2020",
doi = "10.1007/978-1-0716-0290-4_19",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "351--364",
booktitle = "Methods in Molecular Biology",
}