CRISPR/Cas9 Guide RNA Design Rules for Predicting Activity

Kasidet Hiranniramol, Yuhao Chen, Xiaowei Wang

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

A critical stage in performing gene editing experiments using the CRISPR/Cas9 system is the design of guide RNA (gRNA). In this chapter, we conduct a review of the current gRNA design rules for maximizing on-target Cas9 activity while minimizing off-target activity. In addition, we present some of the currently available computational tools for gRNA activity prediction and assay design.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages351-364
Number of pages14
DOIs
StatePublished - Jan 1 2020

Publication series

NameMethods in Molecular Biology
Volume2115
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • CRISPR
  • Cas9
  • Genome editing
  • Machine learning
  • sgRNA

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  • Cite this

    Hiranniramol, K., Chen, Y., & Wang, X. (2020). CRISPR/Cas9 Guide RNA Design Rules for Predicting Activity. In Methods in Molecular Biology (pp. 351-364). (Methods in Molecular Biology; Vol. 2115). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-0290-4_19