TY - JOUR
T1 - CRISPR Mediated Biosensing Toward Understanding Cellular Biology and Point-of-Care Diagnosis
AU - Dai, Yifan
AU - Wu, Yanfang
AU - Liu, Guozhen
AU - Gooding, J. Justin
N1 - Publisher Copyright:
© 2020 Wiley-VCH GmbH
PY - 2020/11/16
Y1 - 2020/11/16
N2 - Recent advances in CRISPR based biotechnologies have greatly expanded our capabilities to repurpose CRISPR for the development of biomolecular sensors for diagnosing diseases and understanding cellular pathways. The key attribute that allows CRISPR to be widely utilized is the programmable and highly selective mechanism. In this Minireview, we first illustrate the molecular principle of CRISPR functioning process from sensing to actuating. Next, the CRISPR based biosensing strategies for nucleic acids, proteins and small molecules are summarized. We highlight some of recent advances in applications for in vitro detection of biomolecules and in vivo imaging of cellular networks. Finally, the challenges with, and exciting prospects of, CRISPR based biosensing developments are discussed.
AB - Recent advances in CRISPR based biotechnologies have greatly expanded our capabilities to repurpose CRISPR for the development of biomolecular sensors for diagnosing diseases and understanding cellular pathways. The key attribute that allows CRISPR to be widely utilized is the programmable and highly selective mechanism. In this Minireview, we first illustrate the molecular principle of CRISPR functioning process from sensing to actuating. Next, the CRISPR based biosensing strategies for nucleic acids, proteins and small molecules are summarized. We highlight some of recent advances in applications for in vitro detection of biomolecules and in vivo imaging of cellular networks. Finally, the challenges with, and exciting prospects of, CRISPR based biosensing developments are discussed.
KW - CRISPR
KW - analytical biochemistry
KW - biosensors
KW - in vitro diagnostics
KW - in vivo biosensing
UR - http://www.scopus.com/inward/record.url?scp=85089786193&partnerID=8YFLogxK
U2 - 10.1002/anie.202005398
DO - 10.1002/anie.202005398
M3 - Review article
C2 - 32521081
AN - SCOPUS:85089786193
SN - 1433-7851
VL - 59
SP - 20754
EP - 20766
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
IS - 47
ER -