CRISPR Mediated Biosensing Toward Understanding Cellular Biology and Point-of-Care Diagnosis

Yifan Dai, Yanfang Wu, Guozhen Liu, J. Justin Gooding

Research output: Contribution to journalReview articlepeer-review

167 Scopus citations

Abstract

Recent advances in CRISPR based biotechnologies have greatly expanded our capabilities to repurpose CRISPR for the development of biomolecular sensors for diagnosing diseases and understanding cellular pathways. The key attribute that allows CRISPR to be widely utilized is the programmable and highly selective mechanism. In this Minireview, we first illustrate the molecular principle of CRISPR functioning process from sensing to actuating. Next, the CRISPR based biosensing strategies for nucleic acids, proteins and small molecules are summarized. We highlight some of recent advances in applications for in vitro detection of biomolecules and in vivo imaging of cellular networks. Finally, the challenges with, and exciting prospects of, CRISPR based biosensing developments are discussed.

Original languageEnglish
Pages (from-to)20754-20766
Number of pages13
JournalAngewandte Chemie - International Edition
Volume59
Issue number47
DOIs
StatePublished - Nov 16 2020

Keywords

  • CRISPR
  • analytical biochemistry
  • biosensors
  • in vitro diagnostics
  • in vivo biosensing

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