CREB and Sp1 regulate the human CD2AP gene promoter activity in renal tubular epithelial cells

Chao Lu; Wei Ren; Xing Ming Su; Jie Qing Chen; Sheng Hua Wu; Xi Rong Guo; Song Ming Huang; Long Hua Chen; Guo Ping Zhou

doi:10.1016/j.abb.2008.03.031

CREB and Sp1 regulate the human CD2AP gene promoter activity in renal tubular epithelial cells

Chao Lu, Wei Ren, Xing Ming Su, Jie Qing Chen, Sheng Hua Wu, Xi Rong Guo, Song Ming Huang, Long Hua Chen, Guo Ping Zhou

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

The human CD2-associated protein (CD2AP) is involved in several molecular signaling pathways and is an important factor responsible for nephrotic syndrome. Here we report the identification of the transcription start point and promoter region of the human CD2AP gene in renal tubular epithelial cells. With luciferase assays and deletion analysis, we found that the region between -558 and -1 bp ahead of the transcription start point is indispensable for the promoter activity of the human CD2AP gene. A CREB site and two Sp1 sites were essential for maintaining the basal transcriptional activity of the human CD2AP promoter. Overexpression of phosphorylated CREB and Sp1 transactivated the human CD2AP promoter, whereas small interfering RNA-mediated blockage of CREB and Sp1 genes expressions inhibited markedly its activity. These findings provide the first analysis of the human CD2AP gene promoter and demonstrate that not only CREB but also Sp1 plays a critical role in regulating basal CD2AP promoter activity in renal tubular epithelial cells.

Original language	English
Pages (from-to)	143-149
Number of pages	7
Journal	Archives of Biochemistry and Biophysics
Volume	474
Issue number	1
DOIs	https://doi.org/10.1016/j.abb.2008.03.031
State	Published - Jun 1 2008

Keywords

cAMP-responsive element-binding protein(CREB)
CD2-associated protein (CD2AP)
Promoter regulation
Stimulating protein 1 (Sp1)
Transcriptional factor

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10.1016/j.abb.2008.03.031

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title = "CREB and Sp1 regulate the human CD2AP gene promoter activity in renal tubular epithelial cells",

abstract = "The human CD2-associated protein (CD2AP) is involved in several molecular signaling pathways and is an important factor responsible for nephrotic syndrome. Here we report the identification of the transcription start point and promoter region of the human CD2AP gene in renal tubular epithelial cells. With luciferase assays and deletion analysis, we found that the region between -558 and -1 bp ahead of the transcription start point is indispensable for the promoter activity of the human CD2AP gene. A CREB site and two Sp1 sites were essential for maintaining the basal transcriptional activity of the human CD2AP promoter. Overexpression of phosphorylated CREB and Sp1 transactivated the human CD2AP promoter, whereas small interfering RNA-mediated blockage of CREB and Sp1 genes expressions inhibited markedly its activity. These findings provide the first analysis of the human CD2AP gene promoter and demonstrate that not only CREB but also Sp1 plays a critical role in regulating basal CD2AP promoter activity in renal tubular epithelial cells.",

keywords = "cAMP-responsive element-binding protein(CREB), CD2-associated protein (CD2AP), Promoter regulation, Stimulating protein 1 (Sp1), Transcriptional factor",

author = "Chao Lu and Wei Ren and Su, {Xing Ming} and Chen, {Jie Qing} and Wu, {Sheng Hua} and Guo, {Xi Rong} and Huang, {Song Ming} and Chen, {Long Hua} and Zhou, {Guo Ping}",

note = "Funding Information: We are grateful to Guntram Suake for the pN3, pN3–CREB and pN3–Sp1 plasmids. We thank the Sanger Institute (Wellcome Trust, Cambridge, UK) for the BAC-clone RP11-385F7. This work was supported by National Natural Science Foundation of China (30570863), Medical Academic Key Talent Program of Jiangsu Province in China (RC2007050), the Medical Grant of Scientific and Technological Development from the Health Department of Jiangsu Province in China (No. K200504), and China Postdoctoral Science Foundation (20070411062).",

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doi = "10.1016/j.abb.2008.03.031",

language = "English",

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T1 - CREB and Sp1 regulate the human CD2AP gene promoter activity in renal tubular epithelial cells

AU - Lu, Chao

AU - Ren, Wei

AU - Su, Xing Ming

AU - Chen, Jie Qing

AU - Wu, Sheng Hua

AU - Guo, Xi Rong

AU - Huang, Song Ming

AU - Chen, Long Hua

AU - Zhou, Guo Ping

N1 - Funding Information: We are grateful to Guntram Suake for the pN3, pN3–CREB and pN3–Sp1 plasmids. We thank the Sanger Institute (Wellcome Trust, Cambridge, UK) for the BAC-clone RP11-385F7. This work was supported by National Natural Science Foundation of China (30570863), Medical Academic Key Talent Program of Jiangsu Province in China (RC2007050), the Medical Grant of Scientific and Technological Development from the Health Department of Jiangsu Province in China (No. K200504), and China Postdoctoral Science Foundation (20070411062).

PY - 2008/6/1

Y1 - 2008/6/1

N2 - The human CD2-associated protein (CD2AP) is involved in several molecular signaling pathways and is an important factor responsible for nephrotic syndrome. Here we report the identification of the transcription start point and promoter region of the human CD2AP gene in renal tubular epithelial cells. With luciferase assays and deletion analysis, we found that the region between -558 and -1 bp ahead of the transcription start point is indispensable for the promoter activity of the human CD2AP gene. A CREB site and two Sp1 sites were essential for maintaining the basal transcriptional activity of the human CD2AP promoter. Overexpression of phosphorylated CREB and Sp1 transactivated the human CD2AP promoter, whereas small interfering RNA-mediated blockage of CREB and Sp1 genes expressions inhibited markedly its activity. These findings provide the first analysis of the human CD2AP gene promoter and demonstrate that not only CREB but also Sp1 plays a critical role in regulating basal CD2AP promoter activity in renal tubular epithelial cells.

AB - The human CD2-associated protein (CD2AP) is involved in several molecular signaling pathways and is an important factor responsible for nephrotic syndrome. Here we report the identification of the transcription start point and promoter region of the human CD2AP gene in renal tubular epithelial cells. With luciferase assays and deletion analysis, we found that the region between -558 and -1 bp ahead of the transcription start point is indispensable for the promoter activity of the human CD2AP gene. A CREB site and two Sp1 sites were essential for maintaining the basal transcriptional activity of the human CD2AP promoter. Overexpression of phosphorylated CREB and Sp1 transactivated the human CD2AP promoter, whereas small interfering RNA-mediated blockage of CREB and Sp1 genes expressions inhibited markedly its activity. These findings provide the first analysis of the human CD2AP gene promoter and demonstrate that not only CREB but also Sp1 plays a critical role in regulating basal CD2AP promoter activity in renal tubular epithelial cells.

KW - cAMP-responsive element-binding protein(CREB)

KW - CD2-associated protein (CD2AP)

KW - Promoter regulation

KW - Stimulating protein 1 (Sp1)

KW - Transcriptional factor

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JO - Archives of Biochemistry and Biophysics

JF - Archives of Biochemistry and Biophysics

IS - 1

ER -

CREB and Sp1 regulate the human CD2AP gene promoter activity in renal tubular epithelial cells

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