Creatine kinase MM isoenzyme subforms in myocardium, cardiac lymph and blood after coronary artery occlusion in dogs

Dana R. Abendschein, Remo L. Morelli, C. Jeffrey Carlson, Bianca Emilson, Elliot Rapaport

Research output: Contribution to journalReview articlepeer-review

13 Scopus citations


Summary: A time-varying pattern of creatine kinase MM (CK-MM) isoenzyme subforms has been found in the blood of patients after acute myocardial infarction, but the site of enzyme modification has not been identified. Therefore, we studied the CK-MM subform patterns in myocardium, cardiac lymph and blood of dogs after coronary artery occlusion. In five conscious dogs, serial blood samples were taken for 72 h after occlusion of the left anterior descending coronary artery. Samples of non-infarcted and infarcted myocardium were taken after 72 h. In five other anaesthetised, open-chest dogs, cardiac lymph and blood samples were taken for 6 h after coronary artery occlusion. CK-MM subforms were quantitated by an isoelectric focusing method. Before coronary occlusion, 64% of the total CK activity in blood appeared as the anodal subform CK-MM 1 (pI 6.3); 20% and 9% as the cathodal subforms CK-MM 2 (pI 6.6) and CK-MM 3 (pI 6.9), respectively. However, after 2 h of coronary occlusion CK-MM 2 and CK-MM 3 were increased (38% and 17% of total activity respectively) compared with CK-MM 1. Between 4 h and 10 h, CK-MM 2 and CK-MM 3 decreased as CK-MM 1 increased restoring the control relative activities of subforms. In contrast to the subform changes in blood, CK-MM 3 was the predominant subform in both non-infarcted and infarcted myocardium after 72 h of coronary occlusion and in cardiac lymph during 6 h of coronary occlusion. Thus, the changing pattern of subforms in blood resulted from conversion of the dominant myocardial subform, CK-MM 3, to other subforms, but not until the enzyme reached the blood. Since the modification of CK-MM was intravascular, we conclude that the pattern of CK-MM subforms in blood may be useful to predict the time of onset of enzyme release from myocardium.

Original languageEnglish
Pages (from-to)690-696
Number of pages7
JournalCardiovascular Research
Issue number11
StatePublished - Nov 1984
Externally publishedYes


  • Acute myocardial infarction
  • Creatine kinase isoenzymes
  • Post-translational enzyme modification


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