COX2/mPGES1/PGE₂ pathway regulates PD-L1 expression in tumor-associated macrophages and myeloid-derived suppressor cells

In recent years, it has been established that programmed cell death protein ligand 1 (PD-L1)-mediated inhibition of activated PD-1⁺ Tlymphocytes plays a major role in tumor escape from immune system during cancer progression. Lately, the anti-PD-L1 and -PD-1 immune therapies have become an important tool for treatment of advanced human cancers, including bladder cancer. However, the underlying mechanisms of PD-L1 expression in cancer are not fully understood. We found that coculture of murine bone marrow cells with bladder tumor cells promoted strong expression of PD-L1 in bone marrow-derived myeloid cells. Tumor-induced expression of PD-L1 was limited to F4/80⁺ macrophages and Ly-6C⁺ myeloid-derived suppressor cells. These PD-L1-expressing cells were immunosuppressive and were capable of eliminating CD8 T cells in vitro. Tumor-infiltrating PD-L1⁺ cells isolated from tumor-bearing mice also exerted morphology of tumor-associated macrophages and expressed high levels of prostaglandin E₂ (PGE₂)-forming enzymes microsomal PGE₂ synthase 1 (mPGES1) and COX2. Inhibition of PGE₂ formation, using pharmacologic mPGES1 and COX2 inhibitors or genetic overexpression of PGE₂-degrading enzyme 15-hydroxyprostaglandin dehydrogenase (15-PGDH), resulted in reduced PD-L1 expression. Together, our study demonstrates that the COX2/mPGES1/PGE₂ pathway involved in the regulation of PD-L1 expression in tumor-infiltrating myeloid cells and, therefore, reprogramming of PGE₂ metabolism in tumor microenvironment provides an opportunity to reduce immune suppression in tumor host.