Cosmid mapping of the human chorionic gonadotropin β subunit genes by field-inversion gel elect rophoresis

Madge Y. Graham; Tetsuo Otani; Irving Boime; Maynard V. Olson; Georges F. Carle; David D. Chaplin

doi:10.1093/nar/15.11.4437

Cosmid mapping of the human chorionic gonadotropin β subunit genes by field-inversion gel elect rophoresis

Madge Y. Graham, Tetsuo Otani, Irving Boime, Maynard V. Olson, Georges F. Carle, David D. Chaplin

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Abstract

A cosmid clone containing the entire hCGβ gene cluster has been isolated. The restriction map of this clone has been determined by an indirect-end-label FIGE (field inversion gel electrophoresis) method. Analysis of this cosmid clone shows that there are 6 hCGβ genes in human genomic DNA. A previously uncloned portion of the hCGβ cluster, termed the "gap" region, has been shovn not to contain any sequences homologous to the hCGβ cDNA. The restriction mapping method employed in this study takes advantage of the superior resolution of FIGE for high molecular weight DNA fragments in the size range 15-50 kb. This method is broadly applicable and permits rapid and accurate restriction mapping for extended regions of genomic DNA that have been cloned into cosmid or lambda vectors.

Original language	English
Pages (from-to)	4437-4448
Number of pages	12
Journal	Nucleic acids research
Volume	15
Issue number	11
DOIs	https://doi.org/10.1093/nar/15.11.4437
State	Published - Jun 11 1987

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abstract = "A cosmid clone containing the entire hCGβ gene cluster has been isolated. The restriction map of this clone has been determined by an indirect-end-label FIGE (field inversion gel electrophoresis) method. Analysis of this cosmid clone shows that there are 6 hCGβ genes in human genomic DNA. A previously uncloned portion of the hCGβ cluster, termed the {"}gap{"} region, has been shovn not to contain any sequences homologous to the hCGβ cDNA. The restriction mapping method employed in this study takes advantage of the superior resolution of FIGE for high molecular weight DNA fragments in the size range 15-50 kb. This method is broadly applicable and permits rapid and accurate restriction mapping for extended regions of genomic DNA that have been cloned into cosmid or lambda vectors.",

author = "Graham, {Madge Y.} and Tetsuo Otani and Irving Boime and Olson, {Maynard V.} and Carle, {Georges F.} and Chaplin, {David D.}",

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AU - Graham, Madge Y.

AU - Otani, Tetsuo

AU - Boime, Irving

AU - Olson, Maynard V.

AU - Carle, Georges F.

AU - Chaplin, David D.

N1 - Funding Information: This study was supported by grants from the Monsanto Company.

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N2 - A cosmid clone containing the entire hCGβ gene cluster has been isolated. The restriction map of this clone has been determined by an indirect-end-label FIGE (field inversion gel electrophoresis) method. Analysis of this cosmid clone shows that there are 6 hCGβ genes in human genomic DNA. A previously uncloned portion of the hCGβ cluster, termed the "gap" region, has been shovn not to contain any sequences homologous to the hCGβ cDNA. The restriction mapping method employed in this study takes advantage of the superior resolution of FIGE for high molecular weight DNA fragments in the size range 15-50 kb. This method is broadly applicable and permits rapid and accurate restriction mapping for extended regions of genomic DNA that have been cloned into cosmid or lambda vectors.

AB - A cosmid clone containing the entire hCGβ gene cluster has been isolated. The restriction map of this clone has been determined by an indirect-end-label FIGE (field inversion gel electrophoresis) method. Analysis of this cosmid clone shows that there are 6 hCGβ genes in human genomic DNA. A previously uncloned portion of the hCGβ cluster, termed the "gap" region, has been shovn not to contain any sequences homologous to the hCGβ cDNA. The restriction mapping method employed in this study takes advantage of the superior resolution of FIGE for high molecular weight DNA fragments in the size range 15-50 kb. This method is broadly applicable and permits rapid and accurate restriction mapping for extended regions of genomic DNA that have been cloned into cosmid or lambda vectors.

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Cosmid mapping of the human chorionic gonadotropin β subunit genes by field-inversion gel elect rophoresis

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